ISSN:
0935-6304
Keywords:
Capillary electrophoresis
;
Electrochemical detection
;
End column detection
;
Nickel electrode
;
Carbohydrates
;
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
Carbohydrates were separated by capillary electrophoresis (CE) and detected electrochemically using a nickel microelectrode which was operated at a constant applied potential (∼0.6 V vs. Ag/AgCI). A simple capillary electrode holder design facilitated alignment between the separation capillary and the working microelectrode without the use of micro-positioning equipment. The separations were performed under alkaline conditions (pH 〉 11), matching the high pH requirements for amperometric detection at the nickel electrode. The analytical procedure developed showed detection limits for the carbohydrates studied in the micromolar range, showing a linear response in the range tested (micromolar to millimolar). The procedure was used to identify sugars in two real samples (i.e., urine and in a common beverage). The potential use of the system for the determination of amino acids was also demonstrated.
Additional Material:
5 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/jhrc.1240191104
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