ISSN:
1052-9306
Schlagwort(e):
Chemistry
;
Analytical Chemistry and Spectroscopy
Quelle:
Wiley InterScience Backfile Collection 1832-2000
Thema:
Chemie und Pharmazie
Notizen:
The combination of a high-performance liquid chromatograph and a magnetic type mass spectrometer with an atmospheric ionization source has been applied for analyses of thermolabile compounds, i.e. antibiotics, amino acids and glycosphingolipids.Chromatography was performed on a 4 mm i.d. x 150 mm column packed with 5 μm ODS at a flow rate of 1 ml min-1. The liquid chromatography/atmospheric pressure ionization (LC/API) magnetic-type mass spectrometer provides not only an abundant pseudo-molecular ion but also fragment ions which give a structural information resulting from collision-induced dissociation at the differential pumping region of the liquid chromatograph/mass spectrometer interface. The isomers of kanamycins could be differentiated from each other on the basis of their mass spectra obtained at the relatively higher drift voltage applied. LC/API mass spectra of leucine and isoleucine were very similar, but it was easy to differentiate one from another by checking the existence of the ions at m/z 43 or m/z 57 in their mass spectra. The LC/API mass spectra of glucosylceramides showed [MH]+ ions of good abundance and significant fragment ions useful for chemical characterization at a proper drift voltage setting in a controlled and reproducible manner.
Zusätzliches Material:
6 Ill.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1002/bms.1200160164
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