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  • 1
    Electronic Resource
    Electronic Resource
    Organelle genome stability in anther-derived doubled haploids of wheat (Triticum aestivum L., cv. ‘Moisson’) (1985)
    Springer
    Theoretical and applied genetics 71 (1985), S. 320-324 
    Details
    ISSN: 1432-2242
    Keywords: Wheat ; Anther-derived doubled haploids ; Chloroplast DNA ; Mitochondrial DNA ; Restriction patterns
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Chloroplast and mitochondrial compartments of a parental line of wheat (Triticum aestivum L., cv. ‘Moisson’) and its anther-derived doubled haploid lines have been analyzed and compared on the basis of their DNA restriction patterns. The results obtained show that no noticeable difference can be detected between doubled haploid lines and parental line at the level of ctDNA and mtDNA organization. It may be concluded that in vitro culture by itself does not systematically generate a cytoplasmic variation in germ cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00252074
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Ribosomal DNA as a convenient probe to follow segregation and possible divergency from expected homozygosity after haploidization of an androgenetic process (1988)
    Springer
    Theoretical and applied genetics 75 (1988), S. 389-396 
    Details
    ISSN: 1432-2242
    Keywords: Ribosomal DNA ; Restriction fragment length polymorphism ; Anther-derived doubled haploids ; Gametoclonal variation ; Wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Restriction fragment length polymorphism of the wheat nuclear ribosomal DNA has been studied in several steps of a breeding scheme, including parental genotypes, F1 hybrid, F9 generation, and anther-derived doubled haploid lines obtained from F9. Ribosomal DNA represents a suitable molecular marker in following segregation and possible divergency from expected homozygosity after haploidization of an androgenetic process. It has been shown to undergo variations among the first cycle-doubled haploid lines in the relative amount of two different sizes of ribosomal DNA repeat units. The specificity and peculiar properties of the plant system used allowed us to assign an intrachromosomal location (short arm of the chromosomes 1B, 1R or 6B) to several ribosomal DNA repeat units that differ by the length of their nontranscribed spacer region.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00276740
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Gametoclonal variation detected in the nuclear ribosomal DNA from doubled haploid lines of a spring wheat (Triticum aestivum L., cv. ‘César’) (1987)
    Springer
    Theoretical and applied genetics 74 (1987), S. 31-37 
    Details
    ISSN: 1432-2242
    Keywords: Wheat ; Anther-derived doubled haploids ; Gametoclonal variation ; Ribosomal DNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The organization of the nuclear ribosomal DNA from a parental line of wheat (Triticum aestivum L., cv. ‘César’) and its anther-derived first cycle and second cycle doubled haploid lines has been analyzed by DNA-DNA molecular hybridization. Restricted DNA has been probed by three subclones of wheat nuclear ribosomal DNA covering the entire repeat unit. No significant difference was detected in the extent of methylation of ribosomal DNA of the doubled haploid lines with respect to the parental line. On the other hand, a variation has been found in the organization of the nontranscribed spacer region of ribosomal DNA of the first cycle doubled haploid line. This variation remains stable after a second cycle of in vitro androgenesis. However, one out of five second cycle doubled haploid lines so far tested showed an additional hybridization band present in the parental line but lacking in the first cycle doubled haploid line.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00290079
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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