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  • 1
    ISSN: 1432-2048
    Keywords: Anthocyanin biosynthesis ; Anthocyanin-3′,5′-methyltransferase ; Petunia (anthocyanin)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Four S-adenosyl-l-methionine:anthocyanin-3′,5′-O-methyltransferases in flowers of Petunia hybrida were separated using the chromatofocusing technique. Each methyltransferase is controlled by one of the methylation genes Mt1, Mt2, Mf1 or Mf2. Molecular weight, pH-activity optimum, isoelectric point, several kinetic properties and the behaviour in the presence of Mg2+, ethylenediaminetetraacetic acid and S-adenosyl-l-homocysteine of each of the four enzymes were determined. The methylation in vitro of delphinidin 3-(p-coumaroyl)-rutinosido-5-glucoside reflected the accumulation patterns of methylated anthocyanins in vivo and established the regulatory role of methyltransferases in vivo.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-2242
    Keywords: Petunia hybrida ; Anthocyanin biosynthesis ; Hydroxylases ; Methyltransferases
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The relation between four methylation genes (Mt1, Mt2, Mf1 and Mf2) in flowers of Petunia hybrida and anthocyanin-methyltransferase activity was investigated in vitro. All genes controlled methyltransferase activity. This activity was measured with cyanidinnd petunidin-derivatives as substrates. A cross provided evidence that the Mf-genes regulate methyltransferases which are distinct from those controlled by the Mtgenes. Different effects of the two Mf-genes in vivo are shown. The results suggest that the four methylationgenes control four different methyltransferases.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 70 (1985), S. 300-305 
    ISSN: 1432-2242
    Keywords: Anthocyanin biosynthesis ; Flavonoid 3′-hydroxylase ; Genetic control ; Petunia hybrida
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary In flower extracts of defined genotypes of Petunia hybrida, an enzyme activity was demonstrated which catalyses the hydroxylation of naringenin and dihydrokaempferol in the 3′-position. Similar to the flavonoid 3′-hydroxylases of other plants, the enzyme activity was found to be localized in the microsomal fraction and the reaction required NADPH as cofactor. A strict correlation was found between 3′-hydroxylase activity and the gene Ht1, which is known to be involved in the hydroxylation of flavonoids in the 3′-position in Petunia. Thus, the introduction of the 3′-hydroxyl group is clearly achieved by hydroxylation of C15-intermediates, and the concomitant occurrence of the 3′,4′-hydroxylated flavonoids quercetin and cyanidin (paeonidin) in the presence of the functional allele Ht1 is due to the action of one specific hydroxylase catalysing the hydroxylation of common precursors for both flavonols and anthocyanins.
    Type of Medium: Electronic Resource
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