ISSN:
1612-1112
Keywords:
Miniaturized HPLC
;
Post-Column Extraction Detection
;
Segmented-Flow Open-Tubular Reactors
;
Membrane Phase Separator
;
Anti-Cancer Drugs
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Abstract An on-line, post-column, extraction system suitable for use with miniaturized HPLC (0.7–1 mm i.d. columns) is described. The system consists of a 30° angle segmenting tee, a PTFE extraction coil of variable length and a membrane phase separator. The internal volume of the phase separator is less than 1 μl. The total variance contribution of the system is between 1 and 6 sec2. The determination of the anti-cancer drugs VP 16 and VM 26 in urine by means of reversed-phase HPLC and fluorescence detection, using both on-column preconcentration and the present miniaturized post-column extraction system is presented as an application.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02279454
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