ISSN:
1432-2048
Keywords:
Antirrhinum (embryo-sac isolation)
;
Embryo sac (isolated)
;
Helianthus (embryo-sac isolation)
;
Nicotiana (embryo-sac isolation)
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract A technique has been developed for isolating embryo sacs (ESs) by enzymatic maceration. Ovules were macerated in a mixture of pectinase, cellulase and, in some cases, snailase and pectolyase Y-23. The ovular tissues were removed and the ESs were isolated in toto. Embryo sacs were isolated from both fixed and fresh ovules of Antirrhinum majus L., Helianthus annuus L. and Nicotiana tabacum L. Fluorochromasia by fluorescein diacetate showed that the ESs isolated from fresh ovules were viable. The method has promise for various histochemical and cell-physiological studies and quite possibly also for in-vitro culture of ESs.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00395045
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