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  • 1
    ISSN: 1573-675X
    Keywords: Apoptosis ; CD95 (Fas/APO-1) ; NF-κB/Rel ; T lymphocytes.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The activity of NF-κB/Rel transcription factors can inhibit the apoptosis induced by TNF, UV or cancer therapy drugs in a number of cell types, including human T lymphocytes. Furthermore, the NF-κB/Rel inducer, phorbol-12-myristate-13-acetate (PMA), has been reported to suppress the CD95-induced apoptosis of human T lymphocytes. To verify whether the survival-enhancing effect of PMA required NF-κB/Rel activity, we generated two Jurkat cell sublines (AL.7 and AL.8) transfected with a pCMV4-IκBα construct, and two (AL.3 and AL.5) with the void pCMV4 vector. Compared to wild type, AL.3 and AL.5 cells, the AL.7 and AL.8 sublines displayed markedly lower amounts of NF-κB/Rel nuclear complexes and a reduced expression of a κB-controlled CAT reporter gene after 1 and 4 h of incubation with PMA, respectively. All the five cell types displayed negligible levels of apoptosis when cultured with medium or PMA alone; when stimulated with the mAb CH-11, the AL.7 and AL.8 sublines displayed apoptotic responses only slightly (〈0.5 fold) higher than control cells. On the other hand, the salvage activity of PMA was partially impaired in the AL.7 and AL.8 sublines. PMA inhibited apoptosis by 〉85% in wild type, AL.3 and AL.5 cells and by 〈60% in the AL.7 and AL.8 sublines; the apoptosis percentages in the mAb CH-11 + PMA cultures of the IκBα-transfected cells were 〉4-fold higher than in control cells. We conclude that the inhibition of the CD95-induced apoptosis by PMA relies on both NF-κB/Rel-dependent and -independent mechanisms. The partial contribution of these nuclear factors to the suppression of apoptosis indicates that the NF-κB/Rel activity can influence the extent of the CD95-induced T cell death.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1434-601X
    Keywords: 25.70-z ; 24.30.Cz ; 25.70.Lm
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract γ-rays are measured in coincidence with the dissipative complex fragments from the35Cl +64Ni reaction at 7.7 A MeV. Theoretical γ-ray coincidence spectra are determined through a fitting procedure using the contribution of the excited fragment statistical γ-decay calculated by means of the CASCADE code. Whereas the expected statistical spectra fit very well the data in the energy regionE ν=2 to 8 MeV, above this energy another emission mechanism in addition to the statistical decay has to be considered in order to reproduce the experimental spectra. Simulations based on the Vlasov equation suggest that this emission could come from the decay of dipole strength excited in the intermediate dinuclear system.
    Type of Medium: Electronic Resource
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