ISSN:
1432-203X
Keywords:
Key words Fusicoccin
;
Arabidopsis thaliana
;
Selection method
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract A mutagenised population of seeds of Arabidopsis thaliana was allowed to germinate in the presence of the positively charged aminoglycoside hygromycin (4 μg/ml) and the fungal toxin fusicoccin (5×10–6 m). This hygromycin concentration, which is non-toxic by itself, becomes toxic when used together with fusicoccin, which stimulates cation uptake. Seeds that had germinated after 3–5 days and produced seedlings with green cotyledons were potentially resistant to fusicoccin and were therefore transferred into sterile Magenta vessels containing 1/2-strength Murashige and Skoog medium. This selection procedure is non-destructive, i.e. it allows the recovery of viable seedlings and their growth into adult plants thus permitting direct physiological characterisation.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s002990050567
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