ISSN:
1573-6849
Keywords:
Arctic char
;
fluorescence in situ hybridization
;
heterochromatin
;
lake trout
;
nucleolus organizer region
;
sex chromosomes
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Polymorphism of the nucleolus organizer region (NOR) on the putative sex chromosomes of Arctic char (Salvelinus alpinus) was examined using conventional cytogenetic and molecular techniques. Variation was observed in the number, size and position of rDNA loci on the sex pair. Fluorescence in situ hybridization (FISH) analyses showed that the sex chromosomes of Arctic char lack the repetitive DNA sequences (MboI/BglII family) that are a prominent feature of the sex chromosomes of lake trout (S. namaycush). Southern analyses of genomic DNAs using an rDNA fragment as probe revealed extensive restriction fragment length polymorphism (RFLP) variation among individuals. Despite the presence of variation in all aspects of this rDNA locus, no sex-specific differences were detected. Repetitive DNAs (multicopy rDNA as in Arctic char or tandem repetitive DNA as in lake trout) appear to play important but different roles in the evolution of the sex chromosomes in these species.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1018411417816
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