ISSN:
1432-203X
Keywords:
Key words Poplar
;
Populus
;
Aspen
;
Cottonwood
;
Transformation
;
Agrobacterium
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract We describe a protocol for Agrobacterium tumefaciens-mediated transformation of hybrid cottonwoods (Populus sections Tacamahaca Spach. and Aigeiros Duby). The protocol has allowed routine transformation of several economically important cottonwood hybrids (Populus trichocarpa Torr. & Gray×P. deltoides Bartr. ex. Marsh. and P. deltoides×P. nigra L.) that were previously difficult to transform. The procedure was applied to 11 different hybrid cottonwood genotypes and one P. deltoides genotype using kanamycin as the selection agent. Additional experiments showed a very strong interaction between auxin preculture and the effectiveness of various cytokinins for induction of shoot organogenesis. The data also demonstrated the superiority of Agrobacterium strain EHA105 over C58 and LBA4404 for T-DNA transfer based on transient assays with a reporter gene.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s002990050019
Permalink
