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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 147 (1987), S. 88-91 
    ISSN: 1432-072X
    Keywords: Aspergillus niger ; ATP:citrate lyase ; Citric acid accumulation ; Lipid biosynthesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract ATP:citrate lyase (EC 4.1.3.8) has been identified in cell-free extracts from the filamentous fungus Aspergillus niger. The enzyme was located in the cytosol. It exhibits an activity at least ten times that of acetate-CoA-kinase (EC 6.2.1.1) during growth on carbohydrates as carbon sources, and is thus considered responsible for acetyl-CoA formation under these conditions. It is formed constitutively and its biosynthesis does not appear to be controlled by changes in the nitrogen or carbon source or type. ATP:citrate-lyase appears to be very labile during conventional purification procedures; a method involving fast protein liquid anion exchange chromatography was thus developed in order to obtain enzyme preparations sufficiently free of enzymes which could interfere with kinetic investigations. This preparation displays commonly known characteristics of ATP:citrate lyase with respect to substrate affinities and cofactor requirements, with the exception that the affinity for citrate is rather low (2.5 mM). No activator was found. The enzyme is inhibited by nucleoside diphosphates, nucleoside monophosphates and palmitoyl-CoA. Regulation of ATP:citrate lyase be the energy charge of the cytosol in relation to lipid or citric acid accumulation is discussed in view of these findings.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    The European physical journal 342 (1992), S. 161-167 
    ISSN: 1434-601X
    Keywords: 24.10.Cn ; 25.70.−z
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract We present a coupled set of equations for the one-body density matrix and the two-body correlation function consistent with trace relations and conservation laws which provide an extension of correlation dynamics on the two-body level. The additional interaction terms are discussed in the context of time-dependent G-matrix theory and NQCD and studied numerically by one-dimensional calculations for colliding finite fermion systems.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    The European physical journal 337 (1990), S. 175-183 
    ISSN: 1434-601X
    Keywords: 24.10.Cn ; 25.70.−z
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract The dynamical description of strongly interacting finite Fermi systems is based on coupled equations of motion for the one-body density matrixρ(11′) and the two-body correlation functionc 2(12, 1′2′) as obtained from the density-matrix hierarchy. The truncation schemes considered exceed the conventional Brueckner-Hartree-Fock scheme and also apply for nonstationary problems. In the limit of slow processes in time the equation of motion for the two-body correlation functionc 2 can be integrated in time and closed expressions can be given for the dynamical evolution ofρ(11′). When performing a Wigner transformation and adopting semiclassical limits this gives a transport equation of the Uehling-Uhlenbeck type for the one-body phase-space distributionf(x,p;t). Furthermore, in the small amplitude limit, we obtain a set of coupled equations for particle-hole (p-h),2p-2p, 2h-2h, 2p-2h, 1p-3h, 1h-3p amplitudes beyond the level of second RPA which provide a genuine basis for the description of giant resonances and their damping width.
    Type of Medium: Electronic Resource
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