ISSN:
1432-0533
Keywords:
Adult human
;
Oligodendrocyte
;
Astrocyte
;
Tissue culture
;
Cryopreservation
;
Bulk isolation
;
Immunofluorescence microscopy
;
Cell type-specific markers
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary Human adult oligodendrocytes were bulkisolated from the brains obtained at autopsy by the Percoll density gradient method and cultured in vitro for 2–28 days. Cultured human oligodendrocytes were then scraped from the dishes or coverslips, frozen at −70°C in the presence of 10% dimethyl sulfoxide as a cryoprotectorant, and then stored in liquid nitrogen tank. After the fast thawing, 55%–70% of human oligodendrocytes plus astrocytes were found to survive in the culture. Identification of cell types was established by using antibodies directed against galactocerebroside (oligodendrocyte) and glial fibrillary acidic protein (astrocyte).
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00695582
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