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Chromosome analysis by fluorescence in situ hybridization of callus-derived regenerants in Allium cyaneum R. (1998)

Lee, S. H. ; Ryu, J. A. ; Do, G. S. ; [et al.]

Springer

Plant cell reports 18 (1998), S. 209-213

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ISSN: 1432-203X

Keywords: Key words Somaclonal variation ; Allium cyaneum ; Fluorescence in situ hybridization ; Autotetraploid regenerants

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract Investigations were performed to confirm the optimal in vitro culture condition for callus induction and plant regeneration, to observe if somoclonal variation occurs among regenerated plants at the ploidy level and to analyse the chromosomal location of 5S and 18S-26S rRNA gene families using fluorescence in situ hybridization in callus-derived plants of Allium cyaneum. High-est callus initiation was achieved with bulb explants cultured on MS medium supplemented with 2,4-D and BAP at 1 mg l–1 each. A total of 195 plants was obtained when using MS medium supplemented with 1 mg l–1 NAA and 5 mg l–1 BAP; about 92% were diploid having 2n=16; 8% showed a variation in ploidy level. Using digoxigenin-labelled 5S rRNA and biotin-labelled 18S-26S rRNA gene probes, we compared the fluorescence in situ hybridization patterns of autotetraploid plants with the A. cyaneum wild type. The 5S rRNA gene sites were detected on the interstitial region in the short arm of chromosome 4 and on the interstitial region in both arms of chromosome 7. The 18S-26S rRNA gene sites were detected on the terminal region of the short arm, including the satellite of chromosome 5, as well as on a part of chromosome B. The chromosomal location of both rRNA genes in regenerated autotetraploid plants corresponded to those of the wild species.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/s002990050558

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Effect of intramolecular and intermolecular exchange processes on the 1H NMR spectra of 2-chloro-1,3,2-dioxarsolanes (1987)

Song, S. K. ; Watkins, C. L. ; Krannich, L. K.

Chichester : Wiley-Blackwell

Organic Magnetic Resonance 25 (1987), S. 484-488

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ISSN: 0749-1581

Keywords: 1H NMR ; 13C NMR ; 2-Chloro-1,3,2-dioxarsolanes ; Intra- and inter-molecular exchange ; Chemistry ; Analytical Chemistry and Spectroscopy

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Chemistry and Pharmacology

Notes: The 1H and 13C NMR spectra of solutions of 2-chloro-4,4,5,5-tetramethyl-1,3,2-dioxarsolane (1) and 2-chloro-1,3,2-dioxarsolane (2) are reported as a function of temperature, concentration and solvent. These data indicate rapid intramolecular conformational averaging for (1) and (2) under all the conditions investigated. Additionally, intermolecular conformational averaging processes are shown to be present. The kinetic parameters for intermolecular exchange were obtained from line shape analysis of the 1H NMR data. These results indicate the intermolecular exchange to be bimolecular in nature, involving a four-centered transition state. The O—CH2—CH2-O torsional angle of (2) has been calculated from the analysis of its AA′BB′ 1H NMR spectral patterns, obtained at low concentrations in the absence of intermolecular exchange.

Additional Material: 2 Ill.