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The role of the epidermis in auxin-induced and fusicoccin-induced elongation of Pisum sativum stem segments (1980)

Brummell, David A. ; Hall, J. L.

Springer

Planta 150 (1980), S. 371-379

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ISSN: 1432-2048

Keywords: Auxin ; Cell elongation ; Epidermis peeling ; Fusicoccin ; Pisum

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract The effects of peeling and wounding on the indole-3-acetic acid (IAA) and fusicoccin (FC) growth response of etiolated Pisum sativum L. cv. Alaska stem tissue were examined. Over a 5 h growth period, peeling was found to virtually eliminate the IAA response, but about 30% of the FC response remained. In contrast, unpeeled segments wounded with six vertical slits exhibited significant responses to both IAA and FC, indicating that peeling does not act by damaging the tissue. Microscopy showed that the epidermis was removed intact and that the underlying tissue was essentially undamaged. Neither the addition of 2% sucrose to the incubation medium nor the use of a range of IAA concentrations down to 10-8 M restored IAA-induced growth in peeled segments, suggesting that lack of osmotic solutes and supra-optimal uptake of IAA were not important factors over this time period. It is concluded that, although the possibility remains that peeling merely allows leakage of hydrogen ions into the medium, it seems more likely that peeling off the epidermis removes the auxin responsive tissue.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00390172

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Anionic polymerization to high vinyl polybutadiene (1981)

Halasa, Adel F. ; Lohr, D. F. ; Hall, J. E.

New York : Wiley-Blackwell

Journal of Polymer Science: Polymer Chemistry Edition 19 (1981), S. 1357-1360

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ISSN: 0360-6376

Keywords: Physics ; Polymer and Materials Science

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Chemistry and Pharmacology

Notes: 1,3-Butadiene was polymerized anionically to polybutadiene which contained up to 100% 1,2-addition product. The atactic 100% 1,2-polybutadiene was prepared with n-butyllithium modified with bis piperidino ethane. The polymerization was done in hexane solvent at -5 to +20°C polymerization temperature.

Additional Material: 2 Tab.