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Digitale Medien

A cortical granule-specific enzyme, B-1,3-glucanase, in sea urchin eggs (1987)

Wessel, Gary M. ; Truschel, Margaret R. ; Chambers, Scott A. ; [weitere]

New York, NY : Wiley-Blackwell

Gamete Research 18 (1987), S. 339-348

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Details

ISSN: 0148-7280

Schlagwort(e): B-1,3-glucanase ; cortical granules ; hyaline layer ; Life and Medical Sciences ; Cell & Developmental Biology

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Biologie

Notizen: The ultrastructural localization of B-1,3-glucanase in three species of sea urchin eggs was determined using a monospecific antibody in an electronmicroscopic immunogold procedure. In all three species, Lytechinus variegatus, Strongylocentrotus purpuratus, and Arbacia punctulata, B-1,3-glucanase was localized specifically to the cortical granules. No other organelle within the egg contained significant label. During the fertilization reaction, B-1,3-glucanase was released from cortical granules into the perivitelline space and became associated with the hyaline layer. No significant label was found in association with the fertilization envelope.

Zusätzliches Material: 4 Ill.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/mrd.1120180408

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