ISSN:
1617-4623
Keywords:
Escherichia coli
;
Bacteriophage λ
;
DNA loops
;
DNA repair
;
Gene conversion
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Unmethylated DNA heteroduplexes with a large single stranded loop in one strand have been prepared from separated strands of DNA from two different strains of bacteriophage λ, one of which has a ∼800 base pair IS1 insertion in the cI gene. The results of transfections with these heteroduplexes into wild-type and mismatch repair deficient bacteria indicate that such large non-homologies are not repaired by the Escherichia coli mismatch repair system. However, the results do suggest that some process can act to repair such large non-homologies in heteroduplex DNA. Transfections of a series of recombination and excision repair deficient mutants suggest that known excision or recombination repair systems of E. coli are not responsible for the repair. Repair of large non-homologies may play a role in gene conversion involving large insertion or deletion mutations.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00326556
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