ISSN:
1432-0878
Keywords:
Colchicine
;
Human enterocyte
;
Glycoprotein transport
;
Autoradiography
;
Biochemistry
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Summary The effect of colchicine on the intracellular transport of 3H-fucose-labelled glycoproteins in the absorptive cells of cultured biopsy specimens of the human intestine was investigated by light- and electron-microscopical autoradiography and by biochemical methods. The results showed a decrease in the radioactivity of the cell coat on the microvilli and an increase in the Golgi apparatus and in the apical vesicles and tubules. This divergence is attributed to a colchicine-induced impairment of the normal transport of cell-coat glycoproteins from the Golgi apparatus, via the apical vesicles and tubules, to the apex of the cell. The radioactivity of the lysosome-like bodies in the absorptive cells cultured with colchicine also increased. This finding supports a crinophagic function of these organelles in the degradation of excess cell-coat material. The investigations were supported in part by the Foundation for Medical Research (FUNGO), which is subsidized by the Netherlands Organisation for the Advancement of Pure Research
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00236244
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