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  • 1970-1974  (6)
  • Biochemistry and Biotechnology  (4)
  • Analytical Chemistry and Spectroscopy  (2)
  • 1
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 14 (1972), S. 915-938 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The integro-differential growth model of Eakman, Fredriekson, and Tsuehiya has been employed to fit cell size distribution data for Schizosaccharomyces pombe grown in a chemostat under severe product inhibition by ethanol. The distributions were obtained with a Coulter aperture and an electronic system patterned after that of Harvey and Marr. Four parameters - mean cell division size, cell division size standard deviation, daughter cell size standard deviation, and a growth rate coefficient - were calculated for models where the cell growth rate was inversely proportional to size, constant, and proportional to size. A fourth model, one where sigmoidal growth behavior was simulated by two linear growth segments, was also investigated. Linear and sigmoidal models fit the distribution data best. While the mean cell division size remained relatively constant at all growth rates, standard deviation of division size distribution increased with increasing holding times. Standard deviation of the daughter size distribution remained small at all dilution rates. Unlike previous findings with other organisms, the average cell size of Schizosaccharomyces pobme increased at low growth rates.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 14 (1972), S. 75-92 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The bacterium Acetobacter suboyxdans and the yeast Saccharomyces carlsbergensis have been grown together on a synthetic medium in a chemostat. Mannitol, the only carbon source fed to the fermenter, is oxidized by the bacteria to fructose. The yeast, which cannot attack mannitol, breaks down the fructose nearly completely. Eight steady states and five transitory periods after changes in flow rate have been analyzed to study the kinetics of the mixed culture. Separate cell concentrations were determined by a modified Coulter counter apparatus. Both sugars were monitored. Both bacteria and yeast may be modeled using Monod's equation, the latter with some deviations. The yeast is unable to grow beyond the washout point of the bacteria, even though its maximum growth rate is much higher. The yield of both organisms decreases with increasing dilution rate, as does their average cell size. After step changes in dilution rate, repeated oscillations of both sugar and cell concentrations usually occur before steady-state conditions are reattained. They are generally in phase, with no definite sign of a lag. Oscillations of yeast and fructose concentrations are more pronounced. Periods average about 6 hr and are not correlated with fermentation conditions or equipment variables. Repeated oscillations are not found after step-downs in pure cultures of A. suboxydans, leading to the conclusion that the instability in mixed cultures may be caused by a feedback mechanism from the yeast to the bacteria.
    Additional Material: 12 Ill.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 16 (1974), S. 1373-1392 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The response of two-species commensalistic systems in a chemostat has been investigated after perturbations in steady state conditions and after step changes in dilution rate. The system is inherently stable with not more than three overshoots and undershoots possible. More complicated commensalistic systems are less stable, with limit cycle response occurring after dilution rate changes when feedback inhibition and feedforward activation occurs. In general variation of feedback parameters is more effective in changing the behavior of the systems than variation of feedforward parameters. Limited agreement with the experimental data of Chao and Reilly was obtained.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 16 (1974), S. 1507-1516 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Ribulose-1,5-diphosphate carboxylase from spinach has been bound to arylamine porous glass with a diazo linkage and to alklamine porous glass with glutaraldehyde. Stability at elevated temperatures and responses to changes of pH and ribulose-1,5-diphosphate, Mg2+, and dithiothreitol concentrations were not significantly different from the soluble enzyme, though stability at 4°C was somewhat improved.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Chichester : Wiley-Blackwell
    Organic Magnetic Resonance 6 (1974), S. 126-127 
    ISSN: 0030-4921
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The observation of long range (10-14 bond) spin-spin coupling between fluorine nuclei (which are very far apart in space) is used to provide information about electronic interactions in bridged dinuclear aromatic compounds.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 3 (1970), S. 1307-1315 
    ISSN: 0030-493X
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The low resolution mass spectra of α-dimethylaminoacetophenone and γ-dimethylaminobutyrophenone are compared to those of δ-methylhexanophenone and dimethyl n-butylamine as well as to those of α-dimethylaminoacetonitrile and γ-dimethylaminobutyronitrile. Analysis indicates that significant fractions of the fragmentations of the aminoketones are directed by the carbonyl. In particular, McLafferty rearrangement of the γ-aminoketone is favored over α-cleavage from nitrogen at ionizing voltages below 15 eV. Although the overall fragmentation patterns are presumably determined both by the distribution of charge and by the relative rates of the fragmentation processes available to the two ionized groups, the intrinsic rates for α-cleavage at ionized nitrogen and at ionized carbonyls appear to be very similar. Kinetic analysis suggests that 26% of the charge resides on the carbonyl in the γ-aminoketone molecular ion. The extent of delocalization of charge in any aminoketone may be partially controlled by the extent of spatial overlap of the two groups. Correlations of mass spectral behavior with photochemical behavior are thus limited because electronic excitation is more localized in the lowest excited states of molecules than charge is in electron-impact produced molecular ions.
    Additional Material: 4 Tab.
    Type of Medium: Electronic Resource
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