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  • Biochemistry and Biotechnology  (2)
  • acid deposition  (2)
  • 1
    Digitale Medien
    Digitale Medien
    Springer
    Plant and soil 140 (1992), S. 249-254 
    ISSN: 1573-5036
    Schlagwort(e): acid deposition ; hardwood forests ; nutrient leaching ; soil inputs ; stemflow ; succession
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract Stemflow and throughfall from a regenerating (8-year-old) southern Appalachian hardwood forest were collected to examine the relative importance of tree bole nutrient leaching in response to acid deposition. Samples from nine (2 m2) stemflow collection plots were analyzed for four dormant season and 11 growing season rainstorm events. Results showed that, relative to throughfall fluxes, stemflow accounted, on average, for approximately 8.5% of total water reaching the forest floor during both dormant and growing season storms. Relative to foliar leaching, K-, SO4-, and PO4 ions appear to be the most easily leached ions from young tree stems. Proportional nitrate and base cation stemflow fluxes increased significantly (p〈0.05) with growing-season storm-event duration, suggesting that the stemsurface nutrient pool is depleted by precipitation more slowly than the foliar pool. On average, proportional stemflow fluxes of SO4 (12%) and K (14%) were consistently higher than reported maximum values for more mature forest stands, which indicates that small-scale stemflow inputs of ions such as these to the forest floor may be important in early successional ecosystems.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 2
    ISSN: 1573-515X
    Schlagwort(e): acid deposition ; forest cover ; forest structure ; biogeochemistry
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie , Geologie und Paläontologie
    Notizen: Abstract The Regional Integrated Lake-Watershed Acidification Study (RILWAS) was conducted to identify and to quantify the environmental factors controlling surface water chemistry in forested watersheds of the Adirondack region of New York. The RILWAS vegetation research was designed to: (1) compare the quantitative patterns of forest cover and tree community structure in the study catchments of the Moose River drainage system; and (2) identify important vegetation differences among study watersheds that might help to explain inter-watershed differences in water chemistry and aquatic responses to acidic deposition. Field transect data indicated that the overall drainage system includes 50% mixed forest cover, 38% hardwood forest, 10% coniferous forest, and 2% wetland cover. Major tree species include yellow birch, red spruce, American beech, sugar maple, eastern hemlock, and red maple. Analysis of forest structure indicated that mean weighted basal area estimates ranged two-fold from 24–48 m2ha−1 among watersheds. Likewise, mean weighted estimates for aboveground biomass and aboveground annual productivity ranged among watersheds from 160 to 320 MT ha−1 and from 8 to 18 MT ha−1 yr−1, respectively. Results showed that differences in surface water chemistry were independent of vegetation differences among watersheds.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 3
    Digitale Medien
    Digitale Medien
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 48 (1995), S. 25-35 
    ISSN: 0006-3592
    Schlagwort(e): DNA purification ; DNA-binding fluorophore ; Fluorescent ligand ; Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: The fluorescent probe PO-PRO-3 was investigated as a potential ligand for the affinity immobilization and purification of genomic or plasmid DNA fragments. Affinities and mechanisms for PO-PRO-3 binding to superhelical and linearized pUC 18 plasmid DNA were examined through measurement of binding isotherms, continuous-variation analysis, and DNA titrations. In addition, the effects of DNA conformation, protein and RNA contaminants, solvent polarity, and ionic strength are examined with the aim of optimizing binding and elution conditions and of assisgning limits to the range of applicability of the affinity purification. © 1995 John Wiley & Sons, Inc.
    Zusätzliches Material: 11 Ill.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 4
    Digitale Medien
    Digitale Medien
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 57 (1998), S. 686-693 
    ISSN: 0006-3592
    Schlagwort(e): NMR spectroscopy ; enzyme hydration ; organic solvents ; subtilisin ; Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Multinuclear NMR spectroscopy has been used to study water bound to subtilisin Carlsberg suspended in tetrahydrofuran (THF), with the water itself employed as a probe of the hydration layer's physicochemical and dynamic characteristics. The presence of the enzyme did not affect the intensity, chemical shift or linewidth of water (up to 8% v/v) added to THF, as measured by 17O- and 2H-NMR. This finding suggests that hydration of subtilisin can be described by a three-state model that includes tightly bound, loosely bound, and free water. Solid-state 2H-NMR spectra of enzyme-bound D2O support the existence of a non-exchanging population of tightly bound water. An important implication is that the loosely-bound water is the same as free water from an NMR viewpoint. This loosely bound water must also be the water responsible for the large increase in catalytic activity observed in previous hydration studies. © 1998 John Wiley & Sons, Inc. Biotechnol Bioeng 57: 686-693, 1998
    Zusätzliches Material: 6 Ill.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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