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  • 1
    ISSN: 0006-3592
    Keywords: nitrification ; immobilized cells ; activation energy ; diffusion limitation ; temperature ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Activation energies of suspended and immobilized nitrifying bacteria were determined and compared to determine if diffusion limitation results in decreased sensitivity for temperature. The activation energy for the respiration activity of suspended Nitrosomonas europaea and Nitrobacter agilis was found to be 86.4 and 58.4 kJ mol-1, respectively. The activation energy for oxygen diffusion in the support material, κ-carrageenan, determined from the effect of temperature on the effective diffusion coefficient (D), was 17.2 kJ mol-1. Consequently, the apparent actvation energy of diffusion limited cells should be lower. It was indeed shown that due to the effect of diffusion limitation and to temperature effects on the Monod constant Ks, the immobilized-cell activity was less sensitive to temperature. The apparent activation energy for immobilized Ns. europaea was between 28.6 and 94.2 kJ mol-1 and for immobilized Nb. agilis between 1.4 and 72.9 kJ mol-1, depending on the oxygen concentration and temperature. © 1995 John Wiley & Sons, Inc.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
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