ISSN:
0173-0835
Keywords:
Capillary electrophoresis
;
Ibuprofen
;
Chiral separation
;
Dextrin 10
;
Heptakis (2,3,6-tri-O-methyl)-β-cyclodextrin
;
Chemistry
;
Biochemistry and Biotechnology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
A capillary electrophoresis method for determination of the enantiomers of ibuprofen and its major phase I metabolites: 2′-hydroxyibuprofen and 2′-carboxyibuprofen in urine samples have been developed. Cyclodextrins and linear dextrins have been investigated as chiral selectors. Simultaneous chiral separation of the enantiomers of ibuprofen, 2′-hydroxyibuprofen and 2′-carboxyibuprofen was obtained using a mixture of dextrin 10 and heptakis (2,3,6-tri-O-methyl)-β-cyclodextrin in a 2-[N-morpholino]ethanesulphonic acid buffer, pH 5.26. The electroosmotic flow was reversed using hexadimethrine bromide as a buffer additive. The method can be used for the determination of the free enantiomers of ibuprofen, 2′-hydroxyibuprofen and 2′-carboxyibuprofen as well as for the indirect determination of their glucuronic acid conjugates in urine samples.
Additional Material:
7 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/elps.1150190316
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