Library

feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 11 (1969), S. 1135-1156 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The participation of polyketides in the biogenesis of natural products has long been bolstered by chemical analogies. Many isotopic tracer studies have validated the acetate-polymalonate route, via presumptive extended poly-β-carbonyl intermediates, to a variety of fungal metabolites. Though implicit as antibiotic precursors, the ephemeral polyketides have not been isolated, nor perhaps with the exception of acetoacetate, can oligoketides become incorporated intact into secondary metabolites. However, a prototypical oligoketides in its stable lactone form, methyltriacetic lactone (3, 6-dimethyl-l-hydroxy-2-pyrone), has been obtained from the tropolone-producing mold P. Stipitatum. A convenient synthesis of this metabolite, by methylation of triacetic lactone followed by partition chromatographic separation of the resultant positional isomers, has been devised. In an experiment with 14C-formate, it was shown that the hypothetical, enzyme-bound polyketide precursor to methyltriacetic lactone is probably involved in stipitatie arid formation, and that the origin of the “extra” methyl or methyl-derived carbons of both substances arises from the identical “C1” pool. Radioactive tracer experiments concerning the biogenesis of pulvilloric acid, a fairly unstable antibiotic substance produced by P. Pulvillorum, showed that its exocyclic carboxyl is formed following initial methyl transfer, whereas the ring system of the molecule is essentially acetate-polymalonate derived. In order to test the hypothesis that methyl-branched C14 polyketide precursors to pulvilloric acid exist and may become integrated into the fatty acid multienzyme complex, presumptive fatty acid congeners to pulvilloric acid such as. 1-methylmyristie, 4-methyllauric, or 2-methyllauric acids were sought. These substances were, however, absent from the mycelial fatty acid spectrum, as well as from the fatty acid moieties of a crystalline glyceridc mixture obtained from the beer. Alternative approaches to the detection or isolation of polyketides are discussed.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...