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  • Biochemistry and Biotechnology  (2)
  • Biokompatibilität  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Continuous culture system for production of biopolymer levan using erwinia herbicola (1991)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 38 (1991), S. 557-560 
    Details
    ISSN: 0006-3592
    Keywords: levan ; continuous culture ; molecular weight ; Erwinia herbicola ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The optimal production of the fructan biopolymer levan by the bacterium Erwinia herbicola was investigated, including variations in nitrogen, carbon and phosphorous sources, pH, incubation time, culture yields up to 19% by weight produced based on conversion of sucrose as the carbon source when grown in a continuous culture system and processed by tangential flow filtration. Product identity was confirmed with gas chromatography (GC) and 13C nuclear magnetic resonance (NMR). Gel permeation chromatography (GPC) and low-angle laser light scattering (LALLS) determination of the molecular weight of the product showed a significant difference in molecular weight values dependent on the method of analysis. Analysis by GPC resulted in molecular weight one order of magnitude lower than LALLS independent of sample, underscoring the unusual nature of this biopolymer.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260380515
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Biomaterialien – humane Toleranz und Integration (1999)
    Springer
    Der Chirurg 70 (1999), S. 847-857 
    Details
    ISSN: 1433-0385
    Keywords: Key words: Biomaterials ; Biocompatibility ; Process engineering ; Reciprocal technology transfer. ; Schlüsselwörter: Biomaterialien ; Biokompatibilität ; Prozesstechnologien ; wechselseitiger Technologietransfer.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung. Biokompatible Werkstoffe und zugehörige Prozesstechnologien, mit denen eine optimale Strukur- und Oberflächenkompatibilität von Implantaten erreicht werden soll, werden vorgestellt. „Vital-avital composites“ für das „tissue engineering“, Zellkulturmodelle, poröse Keramiken und abbaubare Polymere werden beispielhaft dargestellt. Ein Hauptaspekt liegt auf der Konvertierung von Resultaten der Grundlagenforschung in klinische Anwendungen und auf dem Austausch von Technologien aus dem nichtmedizinischen in den medizinischen Bereich und umgekehrt.
    Notes: Summary. Biomaterials and related process engineering in order to obtain optimal surface and structural biocompatibility of implants and devices are presented. Vital-avital composites for tissue engineering, cell culture models, porous ceramics and degradable polymers are introduced as examples. Emphasis is laid on the conversion of basic research results into clinical applications and on the exchange of technologies from the non-medical into the medical field and vice versa.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001040050734
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Tissue engineering lamb heart valve leaflets (1996)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 50 (1996), S. 562-567 
    Details
    ISSN: 0006-3592
    Keywords: tissue engineering ; synthetic biodegradable matrix ; polyglycolic acid ; polylactic acid ; endothelial cell ; heart valve ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Tissue engineered lamb heart valve leaflets (N - 3) were constructed by repeatedly seeding a concentrated suspension of autologous myofibroblasts onto a biodegradable synthetic polymeric scaffold composed of fibers made from polyglycolic acid and polylactic acid. Over a 2-week period the cells attached to the polymer fibers, multiplied, and formed a tissue core in the shape of the matrix. The tissue core was seeded with autologous large-vessel endothelial cells that formed a monolayer which coated the outer surface of the leaflet. The tissue engineered leaflets were surgically implanted in place of the right posterior pulmonary valve leaflet of the donor lamb while on cardiopulmonary bypass. Pulmonary valve function was evaluated by two-dimensional echocardiography with color Doppler which demonstrated valve function without evidence of stenosis and with only trivial regurgitation under normal physiologic conditions. Histologically, the tissue engineered heart valve leaflets resembled native valve leaflet tissue. © 1996 John Wiley & Sons, Inc.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
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    Paper (German National Licenses)
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