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  • 1
    Digitale Medien
    Digitale Medien
    Expression and biochemical characterisation of recombinant AceA, a bacterial α-mannosyltransferase (1999)
    Springer
    Molecular genetics and genomics 261 (1999), S. 933-940 
    Details
    ISSN: 1617-4623
    Schlagwort(e): Key words Bacterial polysaccharide ; Glycosyltransferase ; Acetan ; Acetobacter xylinum ; Biosynthesis
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Biosynthesis of repeat-unit polysaccharides and N-linked glycans proceeds by sequential transfer of sugars from the appropriate sugar donor to an activated lipid carrier. The transfer of each sugar is catalysed by a specific glycosyltransferase. The molecular basis of the specificity of sugar addition is not yet well understood, mainly because of the difficulty of isolating these proteins. In this study, the aceA gene product expressed by Acetobacter xylinum, which is involved in the biosynthesis of the exopolysaccharide acetan, was overproduced in Escherichia coli and its function was characterised. The aceA ORF was subcloned into the expression vector pET29 in frame with the S·tag epitope. The recombinant protein was identified, and culture conditions were optimised for production of the soluble protein. The results of test reactions showed that AceA is able to transfer one α-mannose residue from GDP-mannose to cellobiose-P-P-lipid to produce α-mannose-cellobiose-P-P-lipid. AceA was not able to use free cellobiose as a substrate, indicating that the pyrophosphate-lipid moiety is needed for enzymatic activity.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s004380051040
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  • 2
    Digitale Medien
    Digitale Medien
    Physical modelling fire performance of wall-covering materials (1993)
    New York, NY [u.a.] : Wiley-Blackwell
    Fire and Materials 17 (1993), S. 293-303 
    Details
    ISSN: 0308-0501
    Schlagwort(e): Chemistry ; Polymer and Materials Science
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Architektur, Bauingenieurwesen, Vermessung , Maschinenbau
    Notizen: A small-scale enclosure was constructed for characterizing the contribution of interior finish wall-lining materials to fire growth in compartments. The enclosure was based upon refinements to a design devised by scientists at the National Institute for Standards and Technology for quarter-scale modelling of room fire tests on interior-finish materials. A large volume of evidence is presented demonstrating how this small-scale enclosure could provide the information about surface flammability, heat release, smoke generation and contribution to fire growth that is required for a reasonable assessment of the fire performance of interior wall-finish materials.
    Zusätzliches Material: 22 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/fam.810170606
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  • 3
    Digitale Medien
    Digitale Medien
    Mass spectrometry of the 11H-dibenzo[c,f][1,2]-diazepine systemPartial support of this work by US Public Health Service Grant CA 08495, National Cancer Institute is gratefully acknowledged.See Ref. 1. (1970)
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 3 (1970), S. 1169-1174 
    Details
    ISSN: 0030-493X
    Schlagwort(e): Chemistry ; Analytical Chemistry and Spectroscopy
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Chemie und Pharmazie
    Notizen: The mass spectra of 3,8-dichloro-11H-dibenzo[c,f] [1,2]diazepine, the corresponding diazepin-11-01 and diazepin-11-one and their dihydro and N-oxide derivatives have been recorded. The most important fragmentation of the molecular ions of all diazepines studied was an initial loss of m/e 28, with further fragmentation depending on the 11-substituent. No general pathway was observed for the corresponding N-oxides and dihydro compounds.
    Zusätzliches Material: 9 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/oms.1210030905
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