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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Molecular genetics and genomics 251 (1996), S. 60-68 
    ISSN: 1617-4623
    Keywords: Idiomorph ; DNA binding ; Mating type ; Bipolaris sacchari ; Cochliobolus heterostrophus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The availability of cloned genes that control sexual reproduction (mating type genes) in higher fungi has allowed us to consider the causes of failure to mate in asexual fungi. We report here that the asexual fungusBipolaris sacchari has a homolog of theMAT-2 gene of its sexual ascomycete relativeCochliobolus heterostrophus. TheB. sacchari MAT-2 sequence is highly similar to that ofC. heterostrophus MAT-2 and, in fact, functions in transgenicC. heterostrophus. Thus, the asexual nature ofB. sacchari is not due to absence or mutation ofMAT. When either of theC. heterostrophus MAT genes was transformed intoB. sacchari, the recipient could neither self nor cross with otherB. sacchari strains, in contrast to transgenicC. heterostrophus strains which can do both. Persistent asexuality ofB. sacchari, in spite of the presence of complementary functionalMAT genes, suggests that this fungus lacks genes other thanMAT which are essential for mating. Notably, the transgenicB. sacchari strains were sometimes able to initiate, but not complete, sexual development in interspecific pairings withC. heterostrophus. Transcript analysis showed that theB. sacchari MAT-2 gene is expressed in transgenicC. heterostrophus and that theC. heterostrophus MAT genes are expressed in transgenicB. sacchari. No transcript of the nativeB. sacchari MAT-2 gene was detected under any growth condition tested.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Molecular genetics and genomics 251 (1996), S. 60-68 
    ISSN: 1617-4623
    Keywords: Key words Idiomorph ; DNA binding ; Mating type ; Bipolaris sacchari ; Cochliobolus heterostrophus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The availability of cloned genes that control sexual reproducti on (mating type genes) in higher fungi has allowed us to consider the causes of failure to mate in asexual fungi. We report here that the asexual fungus Bipolaris sacchari has a homolog of the MAT-2 gene of its sexual ascomycete relative Cochliobolus heterostrophus. The B. sacchari MAT-2 sequence is highly similar to that of C. heterostrophus MAT-2 and, in fact, functions in transgenic C. heterostrophus. Thus, the asexual nature of B. sacchari is not due to absence or mutation of MAT. When either of the C. heterostrophus MAT genes was transformed into B. sacchari, the recipient could neither self nor cross with other B. sacchari strains, in contrast to transgenic C. heterostrophus strains which can do both. Persistent asexuality of B. sacchari, in spite of the presence of complementary functional MAT genes, suggests that this fungus lacks genes other than MAT which are essential for mating. Notably, the transgenic B. sacchari strains were sometimes able to initiate, but not complete, sexual development in interspecific pairings with C. heterostrophus. Transcript analysis showed that the B. sacchari MAT-2 gene is expressed in transgenic C. heterostrophus and that the C. heterostrophus MAT genes are expressed in transgenic B. sacchari. No transcript of the native B. sacchari MAT-2 gene was detected under any growth condition tested.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
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