ISSN:
1434-0879
Keywords:
Interferon-γ
;
Receptors
;
Bladder cancer
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary Previously we have shown a differential biological response of three human bladder cancer cell lines (RT4, RT112 and MGH-U1) to gamma interferon (IFN-γ). The present study examines the relationship between the biological response and the expression of the interferon-γ receptor on the tumour cell surface. Using a competitive radioligand binding assay and Scatchard analysis, we measured the number and affinity of the IFN-γ receptors on each of the above cell lines. Individual cells from each line expressed large numbers (29,100–41,800) of high-affinity receptors (k d =2.4–3.9×1010M). There was no statistically significant difference in either of these parameters between the three lines. We therfore conclude that the biological response of these bladder lines to IFN-γ does not relate to the number or affinity of its receptor on the plasma membrane of these tumour cells.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00299722
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