ISSN:
1434-0879
Keywords:
Bladder outlet obstruction
;
Mucosa
;
Serosa
;
Rabbit
;
FT-IR
;
ATR
;
Protein secondary structure
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract The biochemical composition of physiologically moist mucosa and serosa of rabbit bladder before and after bladder outlet obstruction was determined by means of FT-IR spectroscopy with the ATR method and second-derivative analysis. A predominantly β-sheet structure was found in the amide I band for mucosa and serosa before and after obstruction, but the random coil structure increased in both obstructed bladder samples. However, the major β-sheet structure associated with some α-helical structure in the amide II band of mucosa and serosa for non-obstructed bladder changed into a predominanly α-helical structure after bladder obstruction. The obstructed bladder serosa was more pronounced. The amount of glycoproteins doubled in the obstructed bladder serosa, but did not change in the bladder mucosa.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00541893
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