ISSN:
1434-0879
Schlagwort(e):
Concanavalin A
;
Lectin receptors
;
Bladder tumor
;
Electron microscopy
;
X-ray microanalysis
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Medizin
Notizen:
Summary We used concanavalin A (con A)-peroxidase-iron dextran-diaminobenzidine (DAB) technique for the electron microscopic detection of con A binding sites on cell membranes. Normal bladder mucosa showed a sparse distribution of con A binding sites with both transmission (TEM) and scanning (SEM) electron microscopy, but bladder tumors showed a higher concentration in the distribution of con A binding sites in proportion to the histopathological grade of transitional cell carcinoma. Quantitative estimation of the con A binding sites was attempted using scanning X-ray pulse analysis of iron elements contained in the reaction complexes. Con A binding sites were quantitatively the smallest in normal mucosa, increasing proportionate to the grade of the bladder tumor. Some specimens were compared by the ferritin-labelled method and the pattern of ferritin conjugates distribution was similar to that seen with the con A-peroxidase-iron dextran method.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1007/BF00257180
Permalink
