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  • 1
    ISSN: 1433-2981
    Keywords: Apoptosis ; Blood cells ; Hepatic haemopoiesis ; Hibernation ; Urodeles
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Morphocytochemical correlation between proliferation (BrdU uptake) of the haemopoietic liver subcapsular tissue and peripheral blood cell characteristics was performed during active (summer) and hibernating (winter) periods in two Urodele species: Triturus carnifex and Triturus alpestris. The significant decrease of the haemopoietic cell proliferation during the hibernation was accompanied by some modifications both at erythrocyte and leucocyte level. This situation was primarily characterised by a hypofunctionality and ageing of these cells with the disappearance of immature elements from the circulation and cell loss by apoptotic mechanisms. The results indicate that the condition of low metabolism during the winter period in these Urodeles involved deep changes in the physiology of haemopoietic tissue and blood circulating cells.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Comparative clinical pathology 1 (1991), S. 220-223 
    ISSN: 1433-2981
    Keywords: Frog hibernation ; Blood cells ; Morphocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Some morphological features (degree of nuclear segmentation in neutrophils and eosinophils), histochemical patterns (DHFR, LDH, G6PDH) and, in addition, the nucleic acid distribution (DNA, RNA with acridine orange) in the peripheral blood cells of Rana esculenta, during the hibernation phase were investigated. All the observed parameters varied significantly in the frog during hibernation in comparison with the active period. The most evident changes were an increase in the nuclear segmentation of the neutrophils and a lower activity of the histochemically demonstrable DHFR and LDH, probably due to cell ageing. These findings suggest that, in hibernating frogs, there is an increase in the life span of the peripheral blood cells as a consequence of a reduced metabolic activity and a slowing of haematopoiesis.
    Type of Medium: Electronic Resource
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