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  • 1
    Electronic Resource
    Electronic Resource
    Movement of neurosecretory product through the anatomical compartments of the neural lobe of the pituitary gland (1975)
    Springer
    Cell & tissue research 156 (1975), S. 483-497 
    Details
    ISSN: 1432-0878
    Keywords: Neurohypophysis ; Neurosecretion ; Neurophysin ; Neurohypophysia hormones ; Electron microscopy ; Autoradiography
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Electron-microscope autoradiographs have been prepared from the neural lobes of the pituitary glands of rats which had received intracisternal injections of [35S] cysteine at various times before gland removal. The rate of appearance and disappearance of autoradiographically demonstrable radioactivity in the neural lobe closely paralleled that previously determined, biochemically, for radioactive hormones and neurophysins. Radioactivity was appreciably associated with the undilated parts of neurosecretory axons only during the first few hours after injection of the label. The axonal dilations were subdivided into those in which small vesicles could be seen (“endings”) and those in which no small vesicles could be seen (“swellings”). Radioactivity appeared first in “endings” and then in progressively larger and larger profiles of “swellings”. It appeared that newly arrived granules were found close to the limiting membrane of the nerve swelling and that as time progressed they moved deeper and deeper into the swelling. On the basis of the results, suggestions were made for an anatomical explanation of the readily-releasable pool of hormone which has been demonstrated pharmacologically.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00225108
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    The effect of fixation conditions on the ultrastructural appearance of gastrin cell granules in the rat gastric pyloric antrum (1977)
    Springer
    Cell & tissue research 176 (1977), S. 251-263 
    Details
    ISSN: 1432-0878
    Keywords: Gastrin cells ; Electron microscopy ; Fixation ; Granule maturation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The ultrastructural appearance of gastrin cell (G cell) granules was studied after different fixation procedures. When the pH of prefixation was varied there was greater preservation of the electron density of granule cores after acidic (pH 5.0 and 6.0) than after neutral or alkaline (pH 7.0 and 8.0) prefixation. Increasing duration of prefixation at pH 7.3 resulted in progressive loss of electron density of the granule core with swelling and occasional rupture of the limiting membrane. In tissues where most granules had been rendered electron lucent by fixation, those granules remaining dense cored were preferentially located close to the Golgi zone. These findings indicate that the electron density of G cell granules is profoundly affected by conditions of fixation, and that immature granules are more resistant to loss of core density than mature granules. They also suggest that the gastrin granule in vivo, like other polypeptide granules, may have a “solid”, osmotically inactive core.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00229466
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Immunophenotypic evidence for distinct populations of microglia in the rat hypothalamo-neurohypophysial system (1995)
    Springer
    Cell & tissue research 280 (1995), S. 665-673 
    Details
    ISSN: 1432-0878
    Keywords: Microglia ; Hypothalamo-neurohypophysial system ; Antigen-presenting cells ; Blood-brain barrier ; Phagocytosis ; Immunohistochemistry ; Rat (Long Evans)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The morphology, distribution and immunophenotype of microglia throughout the adult rat hypothalamo-neurohypophysial system was examined. Four macrophage-associated antibodies (OX-42, F4/80, ED1 and ED2) were used; the expression of major histocompatibility complex antigens was investigated by use of antibodies against OX-6, OX-17 (MHC class II) and OX-18 (MHC class I). Three distinct types of microglia were identified. The first was located in the magnocellular nuclei; these ‘radially branched’ (‘ramified’) microglia had round cell bodies and long branched processes, and were strongly immunoreactive only for OX-42. The second was located outside the blood-brain barrier in the median eminence, pituitary stalk and neurohypophysis often close to blood vessels; these ‘compact’ microglia had irregular cell bodies and shorter processes, and were strongly labelled by OX-42 and F4/80, weakly labelled by OX-18, and generally unlabelled by ED1, ED2, OX-6 and OX-17. The third type was found in small numbers throughout the system at the surface of the neurvous tissue or around blood vessels; these ‘perivascular’ microglia were elongated cells with no branching processes, and were strongly labelled by ED1, ED2, OX-18, OX-6, OX-17 and F4/80 antibodies but showed variable OX-42 immunoreactivity. Cells in a perivascular location were heterogeneous with respect to their immunophenotype. The presence in the normal adult rat hypothalamo-neurohypophysial system of MHC class-II molecules (OX-6 and OX-17) on a sub-set of perivascular microglia suggests that these cells are capable of presenting antigen to T lymphocytes. The microglia, which lie on either side of the blood-brain barrier, are well placed to facilitate interaction between the immune and neuroendocrine systems.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00318369
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    Immunophenotypic evidence for distinct populations of microglia in the rat hypothalamo-neurohypophysial system (1995)
    Springer
    Cell & tissue research 280 (1995), S. 665-673 
    Details
    ISSN: 1432-0878
    Keywords: Key words: Microglia ; Hypothalamo-neurohypophysial system ; Antigen-presenting cells ; Blood-brain barrier ; Phagocytosis ; Immunohistochemistry ; Rat (Long Evans)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. The morphology, distribution and immunophenotype of microglia throughout the adult rat hypo- thalamo-neurohypophysial system was examined. Four macrophage-associated antibodies (OX-42, F4/80, ED1 and ED2) were used; the expression of major histocompatibility complex antigens was investigated by use of antibodies against OX-6, OX-17 (MHC class II) and OX-18 (MHC class I). Three distinct types of microglia were identified. The first was located in the magnocellular nuclei; these ’radially branched’ (’ramified’) microglia had round cell bodies and long branched processes, and were strongly immunoreactive only for OX-42. The second was located outside the blood-brain barrier in the median eminence, pituitary stalk and neurohypophysis often close to blood vessels; these ’compact’ microglia had irregular cell bodies and shorter processes, and were strongly labelled by OX-42 and F4/80, weakly labelled by OX-18, and generally unlabelled by ED1, ED2, OX-6 and OX-17. The third type was found in small numbers throughout the system at the surface of the nervous tissue or around blood vessels; these ’perivascular’ microglia were elongated cells with no branching processes, and were strongly labelled by ED1, ED2, OX-18, OX-6, OX-17 and F4/80 antibodies but showed variable OX-42 immunoreactivity. Cells in a perivascular location were heterogeneous with respect to their immunophenotype. The presence in the normal adult rat hypothalamo-neurohypophysial system of MHC class-II molecules (OX-6 and OX-17) on a sub-set of perivascular microglia suggests that these cells are capable of presenting antigen to T lymphocytes. The microglia, which lie on either side of the blood-brain barrier, are well placed to facilitate interaction between the immune and neuroendocrine systems.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00318369
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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