ISSN:
1432-072X
Keywords:
Azolla
;
Cyanobacteria
;
Blue-green algae
;
Nitrogen fixation
;
Symbiont
;
Photoheterotroph
;
Algal isolation
;
Photosynthesis
;
Plant metabolism
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract A procedure has been developed to isolate cyanobacteria from the aquatic fern Azolla. The method is based upon recovery of cyanobacterial “bundles” from digests of plants and use of this material as a massive inoculum for nitrogen-free media, followed by prolonged incubation in light. The procedure appears to select for those cells capable of growth in vitro. Isolated cyanobacteria were found to resemble Anabaena sp. morphologically but were capable of heterotrophic growth and had high nitrogenase activity when grown on fructose in the dark.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00409103
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