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  • 1
    Electronic Resource
    Electronic Resource
    Competitiveness and communication for effective inoculation byRhizobium, Bradyrhizobium and vesicular-arbuscular mycorrhiza fungi (1994)
    Springer
    Cellular and molecular life sciences 50 (1994), S. 884-889 
    Details
    ISSN: 1420-9071
    Keywords: Bradyrhizobium ; communication ; competitiveness ; Rhizobium ; vesicular-arbuscular (VA) mycorrhiza fungi
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract After a short summary on the ecology and rhizosphere biology of symbiotic bacteria and vesicular-arbuscular (VA) mycorrhiza fungi and their application as microbial inocula, results on competitiveness and communication are summarized. Stress factors such as high temperature, low soil pH, aluminium concentrations and phytoalexins produced by the host plants were studied withRhizobium leguminosarum bv.phaseoli andRhizobium tropici onPhaseolus beans. Quantitative data for competitiveness were obtained by usinggus + (glucoronidase) labelled strains, which produce blue-coloured nodules. ForPhaseolus-nodulating rhizobia, a group specific DNA probe was also developed, which did not hybridize with more than 20 other common soil and rhizosphere bacteria. Results from several laboratories contributing to knowledge of signal exchange and communication in theRhizobium/Bradyrhizobium legume system are summarized in a new scheme, including also defense reactions at the early stages of legume nodule initiation. Stimulating effects of flavonoids on germination and growth of VA mycorrhiza fungi were also found. A constitutive antifungal compound in pea roots, β-isoxazolinonyl-alanine, was characterized.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01923474
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Osteogenesis imperfecta: Biochemical and clinical evaluation of 13 cases (1981)
    Springer
    Journal of molecular medicine 59 (1981), S. 91-93 
    Details
    ISSN: 1432-1440
    Keywords: Collagen synthesis ; Fibroblast cultures ; Osteogenesis imperfecta ; Kollagensynthese ; Fibroblastenkulturen ; Osteogenesis imperfecta
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Hautfibroblasten wurden von 13 Patienten mit Osteogenesis imperfecta gezüchtet und deren Kollagensynthese in vitro untersucht. Dabei fand sich bei 7 Patienten, die durch nur milde Manifestation der Erkrankung charakterisiert waren, eine Störung des Verhältnisses der Kollagentypen I und III. Fibroblasten von solchen Patienten mit einer schweren Form der Osteogenesis imperfecta synthetisierten die Kollagentypen I und III in einem normalen Verhältnis.
    Notes: Summary Skin fibroblasts were cultured from 13 patients with Osteogenesis imperfecta and collagen biosynthesis was investigated in vitro. In those patients characterised by only mild manifestations of the disease, the ratio of collagen types I and III was disturbed. By contrast, fibroblasts obtained from patients with Osteogenesis imperfecta of a more severe type synthesised collagen types I/III in a normal ratio.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01477288
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    A constitutional disorder of connective tissue suggesting a defect in collagen biosynthesis (1974)
    Springer
    Journal of molecular medicine 52 (1974), S. 906-912 
    Details
    ISSN: 1432-1440
    Keywords: Constitutional connective tissue disorder ; fibroblast cultures ; collagen biosynthesis ; Konstitutionelle Bindegewebserkrankung ; Fibroblastenkulturen ; Kollagenbiosynthese
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Es wird über eine konstitutionelle Bindegewebserkrankung bei dem 10 Jahre alten Sohn blutsverwandter Eltern berichtet. Das klinische und röntgenologische Befundmuster unterscheidet sich von bisher bekannten Syndromen, insbesondere der Osteogenesis imperfecta und dem Marfan-Syndrom, mit denen gewisse Ähnlichkeiten bestehen. In Fibroblastenkulturen aus Hautbiopsien wurde die Kollagenbiosynthese untersucht. Dabei zeigte sich, daß die Fibroblasten unterschiedliche Typen von α1-Ketten synthetisieren, während α2-Ketten weder im Kulturmedium noch in Zellextrakten nachgewiesen werden konnten. Es wird vermutet, daß die Störung der Kollagenbiosynthese mit den klinischen und röntgenologischen Befunden der Erkrankung in Verbindung steht.
    Notes: Summary An apparently “new” connective tissue disorder is described in the 10 year old son of consanguineous parents. The patient showed clinical and radiographic abnormalities reminiscent of both osteogenesis imperfecta and Marfan syndrome. Collagen biosynthesis was analysed in fibroblast cultures from skin biopsies. Fibroblasts of the patient synthesized different types ofα1 chains. No α2 chains could be detected in cell extracts or in culture media suggesting a defect in the synthesis of these compounds. The peculiar pattern of clinical abnormalities may be causally related to the apparent defect in collagen synthesis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01468935
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Hopanoid lipids in Bradyrhizobium and other plant-associated bacteria and cloning of the Bradyrhizobium japonicum squalene-hopene cyclase gene (1996)
    Springer
    Plant and soil 186 (1996), S. 107-112 
    Details
    ISSN: 1573-5036
    Keywords: Bradyrhizobium ; hopanoids ; nitrogen fixation ; plant-microbe interaction ; Rhizobium ; squalene-hopene cyclase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Hopanoid lipids have been discovered recently in a number of nitrogen-fixing soil bacteria and in Bradyrhizobium bacteria which fix nitrogen in association with legume plants. We report here an investigation of the hopanoid content in an additional number of soil bacteria capable of living in close association with plants. Of the strains investigated, hopanoids were discovered in phototrophic, nitrogen-fixing bacteria and in an extended number of Bradyrhizobium strains. Strains in which hopanoids so far have not been found belong to the following genera: Rhizobium, Sinorhizobium, Phyllobacterium, Agrobacterium, and Azoarcus. To address the function of hopanoids in Bradyrhizobium, we cloned the gene coding for a key enzyme of hopanoid biosynthesis, the squalene-hopene cyclase, and expressed the gene in E. coli. The recombinant enzyme catalyzed in vitro the cyclization of squalene to hopanoid derivatives.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00035063
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    Paper (German National Licenses)
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