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  • 1
    Electronic Resource
    Electronic Resource
    Pollen stigma interactions in Brassica oleracea (1980)
    Springer
    Theoretical and applied genetics 58 (1980), S. 241-246 
    Details
    ISSN: 1432-2242
    Keywords: Brassica ; Pollen germination ; Pollen surface ; Self-incompatibility ; Stigma surface
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Recent studies on the mechanism of self-incompatibility in Brassica indicate the location, nature and mode of action of the molecules involved. Characteristics of the pollen surface and the stigma surface are described in detail, together with new information pertaining to the recognition molecules located therein. A sequence of events is outlined leading from pollination, through adhesion, hydration, germination, and tube growth to acceptance and ultimate compatibility. The characteristics of rejection of incompatible grains are described for each stage of the pollen-stigma interaction. It is proposed that recognition of proteins from the coating of self-pollen by the molecules in the pellicle results in the formation of a biologically-active complex which inhibits water supply to the incompatible grain, and that all other manifestations of incompatibility are a consequence of this initial response.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00265173
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Pollen-pistil interaction in Brassica oleracea (1979)
    Springer
    Planta 146 (1979), S. 211-216 
    Details
    ISSN: 1432-2048
    Keywords: Brassica ; Pollen adhesion ; Pollen hydration ; Self-incompatibility ; Tryphine mobility
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Quantitative studies of the adhesion of pollen grains to the stigma in Brassica oleracea revealed that self-pollen is initially less firmly bound than cross-pollen. The pollen grain tryphine, believed to be important in the adhesion process, has been shown to differ in mobility following self- and cross-pollination when observed using fluorescent probes. The hydration of the pollen grains has been investigated in vitro by measuring the changes in shape, volume and fresh weight of the imbibing grains. Whilst little change in volume could be detected there was a considerable increase in fresh weight together with a change of shape. The significance of these events, which occur prior to pollen germination, is discussed in relation to their effect upon subsequent germination and expression of self-incompatibility.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00388234
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    A glycoprotein associated with the acquisition of the self-incompatibility system by maturing stigmas of Brassica oleracea (1979)
    Springer
    Planta 146 (1979), S. 179-183 
    Details
    ISSN: 1432-2048
    Keywords: Brassica ; Cellular recognition ; Glycoproteins ; Self-incompatibility
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Iso-electric focusing of extracts derived from stigmatic homogenates of Brassica oleracea reveals that the mature stigma possesses large quantities of a glycoprotein not present in earlier stages of development in the bud. Pollen germination experiments carried out in parallel with the biochemical tests suggest that the appearance of this glycoprotein, which has an isoelectric point of pH 5.8, is coincident with the development of the self-incompatibility response. The site of this protein, and the role it may play in pollen-stigma interactions are discussed in terms of current models of the self-incompatibility system in Brassica.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00388229
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    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    Pollen-stigma interactions in Brassica oleracea; a new pollen germination medium and its use in elucidating the mechanism of self incompatibility (1983)
    Springer
    Theoretical and applied genetics 65 (1983), S. 231-238 
    Details
    ISSN: 1432-2242
    Keywords: Brassica ; Pollen ; Pollen germination ; Germination medium ; Self incompatibility
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A simple medium is described which supports the germination of Brassica pollen in vitro. The method is completely reliable and the percentage germination achieved is comparable to that found on a compatible stigma. The pollen tubes produced attain lengths equal to those growing in the style and exhibit similar growth kinetics. The major difference between this medium and other less-reliable media is the addition of 1 mM Tris. The efficacy of Tris is shown to be due in part to the establishment of an optimum pH but an “amine effect” is also identified. The optimum pH for growth in vitro may be different from that encountered on the stigma. Atmospheric concentration of CO2 and pollen population density are also shown to have pronounced effects on germination in vitro. Germination in vitro is not significantly affected by prior incubation on the stigma surface. Neither crossnor self-stigmas exert any apparent influence on pollen viability in this respect. Similarly the numbers of tube initials produced in atmospheres of 98% relative humidity (RH) are not altered by such treatments. Conversely, incubation in germination medium and in 98% RH prior to pollination appears to modify pollen-stigma interactions to some extent. Stigma extracts added to the germination medium cause an increase in the rate of hydration of both cross- and self-pollen, but this effect is greater in the case of cross-pollen. Evidence for specific inhibition of self-pollen germination by stigma extracts added to the medium was not obtained. The relevance of these phenomena to current concepts of the mechanism of self incompatibility (SI) are discussed and a potential use of the medium in overcoming the SI response is suggested.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00308074
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    Paper (German National Licenses)
    Fulltext
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