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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 71 (1985), S. 145-152 
    ISSN: 1432-2242
    Keywords: Tomato ; Isochromosomes ; Breakage-fusion-bridge-cycle ; Phenotypic instability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Cultivated tomato lines with 7–9 copies of extra chromosomes, which were fully heterochromatic, were found to have normal growth and reproduction. Cytological evidence suggested that all extra chromosomes in the 24 plants investigated were derived from a single isochromosome of the short arm of chromosome 2. In spite of their common origin, the individual extra chromosomes within and between plants varied considerably with respect to size and morphology. The morphological variation of these extra chromosomes was found to result from chromosome-or chromatid breakage-fusion-bridge-cycle (BFBC). In order to verify whether BFBC could induce “genomic shock” and instability, the phenotypes of more than 10,000 seedlings from some of the progeny were examined. There was a 3-to 4-fold increase in the occurrence of the number of chlorophyll variegated sectors in the progeny of plants with BFBC when compared to the control variety.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1617-4623
    Keywords: Key words Tomato ; Alternaria stem canker ; Map-based cloning ; Physical mapping ; Recombination
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The Alternaria stem canker disease of tomato is caused by the necrotrophic fungal pathogen Alternaria alternata f. sp. lycopersici (AAL). The fungus produces AAL toxins that kill the plant tissue. Resistance to the fungus segregates as a single locus, called Asc, and has been genetically mapped on chromosome 3 of tomato. We describe here the establishment of a 1383-kb YAC contig covering the Asc locus and a series of plants selected for recombination events around the Asc locus. It was shown that the YAC contig corresponds to a genetic distance of at least 11.2 cM. Thus, the recombination rate in the Asc region is six times higher (123 kb/cM) than the average for the tomato genome. Furthermore, the Asc locus could be localised to a 91-kb fragment, thus paving the way for the cloning and identification of the Asc gene(s) by complementation.
    Type of Medium: Electronic Resource
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