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Sodium-dependent adenosine transport is diminished in brush border membrane vesicles from hypothyroid rat kidney (1996)

Martínez, F. ; Franco, Martha ; Quintana, Alicia ; [et al.]

Springer

Pflügers Archiv 433 (1996), S. 269-275

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ISSN: 1432-2013

Keywords: Key words Adenosine receptors ; Nucleoside transport ; Sodium-coupled adenosine transport ; Hypothyroidism ; Brush border

Source: Springer Online Journal Archives 1860-2000

Topics: Medicine

Notes: Abstract Studies of the uptake of [3H]adenosine ([3H]ADO) were performed using brush border membrane vesicles (BBMV) from normal (N) and hypothyroid (Tx) rat kidneys, to test if decreased Na+ reabsorption in hypothyroidism might be associated with abnormalities in ADO transport. [3H]ADO uptake (1–10 μmol) for both conditions was measured in the presence of Na+ (10–150 mmol/l); the effects of dipyridamole (10 μmol/l) and 1,3-dipropyl-8-(2-amino-4-chlorophenyl)xanthine (PACPX, 10 μmol/l) were also studied. Na+-stimulated ADO uptake was decreased in Tx BBMV. Michaelis–Menten constants showed a decreased ADO carrier affinity (K m 2.46 ± 0.14 in N, vs K m 4.46 ± 0.88 μmol/l in Tx, P〈0.05), with no change in the number of carriers (V max 295 ± 25 in N, vs 229.2 ± 56 pmol·min–1·mg protein in Tx). Na+ affinity remained unchanged (K Na+ 11.5 ± 3.5 in N, vs K Na+ 12.72 ± 0.7 mmol/l in Tx). Inhibition of Na+-dependent ADO transport was 50% in N as opposed to 58% in Tx with dipyridamole, and 72% in N versus 47% in Tx with PACPX. These results suggest that decreased Na+-dependent ADO cotransport contributes to the diminished tubular reabsorption that occurs in hypothyroidism.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/s004240050277

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Photometric determination of molybdenum in iron and steels using tin(II) chloride in nonaqueous solvent (1973)

Bermejo-Martínez, F. ; Latas-Pérez, P.

Springer

Fresenius' Zeitschrift für analytische Chemie 264 (1973), S. 139-142

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ISSN: 1618-2650

Keywords: Best. von Molybdän in Eisen, Stahl ; Śpektralphotometrie ; Zinn(II)-chlorid als Reduktionsmittel, Thiocyanatkomplex

Source: Springer Online Journal Archives 1860-2000

Topics: Chemistry and Pharmacology

Description / Table of Contents: Summary A new procedure is proposed for the photometric determination of molybdenum in iron and steels using thiocyanate and tin(II) chloride solution [in glycerol-ethannol (3∶1) or in diethylenglycol] as a reducer and extraction with n-butyl acetate. Beer's law is obeyed between 0.2 and 80 μg Mo/ml. The minimum photometric error is 3.2%, the molar absorptivity is 15640 at 470 nm and the best concentration range for determination is between 0.6 and 6 μg Mo/ml.

Notes: Zusammenfassung Das vorgeschlagene Verfahren beruht auf Bildung des Thiocyanatkomplexes unter Verwendung von Zinn(II)-chlorid als Reduktionsmittel [in Glycerin/Äthanol (3∶1) oder Diäthylenglykol]. Als Extraktionsmittel dient n-Butylacetat. Das Beersche Gesetz wird im Bereich 0,2–80 μg Mo/l befolgt. Der kleinste photometrische Fehler beträgt 3,2%, die molare Extinktion 15640 bei 470 nm und der optimale Konzentrationsbereich 0,6–6 μgMo/l.