ISSN:
1573-5044
Keywords:
Buddleia × weyeriana
;
colchicine
;
flow cytometry
;
micropropagation
;
ploidy
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The aim was to produce a tetraploid form of Buddleia globosa to facilitate introgression of yellow flower colour into B. davidii, which is naturally tetraploid. Protocols were established for the micropropagation of B. globosa and tetraploid plants were obtained by application in vitro of colchicine to pre-cultured excised nodal sections. Three concentrations of colchicine were applied (0.01%, 0.05% and 0.1% w/v) for 1, 2 or 3 days. At 0.01% tetraploids were produced only after 2 days of application. All other treatments produced at least one tetraploid. The colchicine technique was extremely effective: of 29 lines tested, 19 were tetraploid and 5 were mixoploid. The vegetative characteristics of these tetraploids are described and the flowering characteristics of the three that flowered.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1006434803003
Permalink
