ISSN:
1432-203X
Keywords:
Key words Genetic transformation
;
C3
;
C4 intermediate species
;
Flaveria pubescens
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The dicot genus Flaveria (Asteraceae), besides species with C3 or C4 photosynthesis, contains taxa with a broad range of different states of transition between the two major photosynthetic types. We have developed a reproducible and efficient Agrobacterium-mediated method for the stable genetic transformation of the C3–C4 intermediate species F. pubescens. Fusion constructs of the reporter gene β-glucuronidase (uidA, GUS) to several plant promoters, mainly derived from genes encoding subunits of the glycine cleavage system (gdcs), have been used to confirm the reproducibility and efficiency of the method. The stable integration of the foreign DNA has been examined by Southern analysis, kanamycin resistance, GUS enzyme activities and histochemical staining. Transformed shoots can be routinely obtained within 8–10 weeks after co-cultivation with A. tumefaciens.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s002990050308
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