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  • 1
    Electronic Resource
    Electronic Resource
    Identification of IL-1β-induced messenger RNAs in rat pancreatic beta cells by differential display of messenger RNA (1999)
    Springer
    Diabetologia 42 (1999), S. 1199-1203 
    Details
    ISSN: 1432-0428
    Keywords: Keywords Beta cells ; chemokine ; phospholipase-D ; DDRT-PCR ; interleukin-1 ; monocyte chemoattractant protein-1 ; adenine nucleotide translocator ; CINC-1 ; CINC-3.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Aims/hypothesis. Interleukin-1β is a putative mediator of pancreatic beta-cell dysfunction and damage in Type I (insulin-dependent) diabetes mellitus. To better understand the molecular mechanisms involved in IL-1β effects, we carried out a differential display of mRNA by RT-PCR to identify novel cytokine-regulated genes. Methods. Fluorescence activated cell sorting-purified rat pancreatic beta-cells were exposed for 6 or 24 h to IL-1β. Differentially expressed cDNA bands were cloned and then identified by comparing their sequences with data from the GenBank. Differential gene expression was confirmed by RT-PCR using specific primers. Results. Interleukin-1β increased the expression of adenine nucleotide translocator-1, phospholipase D-1 and cytokine-induced neutrophil chemoattractant-1 and decreased expression of the protein tyrosine phosphatase-like protein IA-2. Interleukin-1β-induced differential expression of these genes in beta cells was confirmed by RT-PCR. In additional studies, IL-1β was shown to induce chemokines other than cytokine-induced neutrophil chemoattractant-1, including cytokine-induced neutrophil chemoattractant-3 and monocyte chemotactic protein-1. Conclusion/interpretation. Our observations indicate that IL-1β modifies the expression of several genes in pancreatic beta cells. These genes may affect both function, viability and beta-cell recognition by the immune system. Functional characterization of the mRNAs which have been identified could facilitate a better understanding of the mechanisms leading to beta-cell destruction in Type I diabetes. [Diabetologia (1999) 42: 1199–1203]
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001250051292
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    High cell density and productivity culture of Chinese hamster ovary cells in a fluidized bed bioreactor (1999)
    Springer
    Cytotechnology 29 (1999), S. 215-220 
    Details
    ISSN: 1573-0778
    Keywords: CHO ; cytoline microcarrier ; fluidized bed bioreactor (FBR) ; stirred tank bioreactor (STR)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract A recombinant Chinese hamster ovary clone was cultivated in a 2L Cytopilot Mini fluidized bed bioreactor using Cytoline 1 microcarriers and a 10L B. Braun stirred tank bioreactor with Cytodex 1 microcarriers. Cytoline 1 is a macroporous polyethylene microcarrier and Cytodex 1 is a solid DEAE-dextran microcarrier. Cytoline 1 microcarriers in the fluidized bed bioreactor were gently mixed by an uplifting flow. Circulation and sparging in Cytopilot Mini were separated from the fluidized microcarrier bed. Cytopilot Mini bioreactor with Cytoline 1 microcarriers offered 2.3 times more surface area than the stirred tank bioreactor. The 2L fluidized bed bioreactor accommodated approximately half the cells in the 10L stirred tank bioreactor. Moreover, Cytopilot Mini had approximately three times more product output rate and 5.5 times higher specific productivity than the stirred tank bioreactor.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008064217040
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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