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  • 1
    Electronic Resource
    Electronic Resource
    High cell density and productivity culture of Chinese hamster ovary cells in a fluidized bed bioreactor (1999)
    Springer
    Cytotechnology 29 (1999), S. 215-220 
    Details
    ISSN: 1573-0778
    Keywords: CHO ; cytoline microcarrier ; fluidized bed bioreactor (FBR) ; stirred tank bioreactor (STR)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract A recombinant Chinese hamster ovary clone was cultivated in a 2L Cytopilot Mini fluidized bed bioreactor using Cytoline 1 microcarriers and a 10L B. Braun stirred tank bioreactor with Cytodex 1 microcarriers. Cytoline 1 is a macroporous polyethylene microcarrier and Cytodex 1 is a solid DEAE-dextran microcarrier. Cytoline 1 microcarriers in the fluidized bed bioreactor were gently mixed by an uplifting flow. Circulation and sparging in Cytopilot Mini were separated from the fluidized microcarrier bed. Cytopilot Mini bioreactor with Cytoline 1 microcarriers offered 2.3 times more surface area than the stirred tank bioreactor. The 2L fluidized bed bioreactor accommodated approximately half the cells in the 10L stirred tank bioreactor. Moreover, Cytopilot Mini had approximately three times more product output rate and 5.5 times higher specific productivity than the stirred tank bioreactor.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008064217040
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    THE COMPUTATION OF WAVELET-GALERKIN APPROXIMATION ON A BOUNDED INTERVAL (1996)
    Chichester [u.a.] : Wiley-Blackwell
    International Journal for Numerical Methods in Engineering 39 (1996), S. 2921-2944 
    Details
    ISSN: 0029-5981
    Keywords: wavelet orthogonal bases ; wavelet-Galerkin method ; Burgers' equation ; numerical method ; approximation ; Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: This paper describes exact evaluations of various finite integrals whose integrands involve products of Daubechies' compactly supported wavelets and their derivatives and/or integrals. These finite integrals play an essential role in the wavelet-Galerkin approximation of differential or integral equations on a bounded interval.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
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    Paper (German National Licenses)
  • 3
    Electronic Resource
    Electronic Resource
    SHAPE SENSITIVITY ANALYSIS FOR STEADY METAL-FORMING PROCESSES (1996)
    Chichester [u.a.] : Wiley-Blackwell
    International Journal for Numerical Methods in Engineering 39 (1996), S. 1199-1217 
    Details
    ISSN: 0029-5981
    Keywords: sensitivity analysis ; shape optimization ; extrusion ; drawing ; Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: This paper develops a numerical technique for determining the shape sensitivity parameters in steady metal- forming processes such as drawing and rolling. The adjoint method is applied to the discrete non-linear system of equations in the finite element model in order to determine the discrete matrix of sensitivity parameters. In this work, two specific cases are considered. The first case involves determining the sensitivity of the process power requirement to the process geometry and the second case involves determining the sensitivity of the internal state variable distribution in the final product to the process geometry. The process geometry is assumed to be characterized by a finite number of shape parameters. The internal state variable distribution in this case represents the resistance to plastic flow and is considered to be related to the quality of the final product. Numerical examples for a simple drawing process are presented to demonstrate the efficiency of the algorithm.
    Additional Material: 11 Ill.
    Type of Medium: Electronic Resource
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    Paper (German National Licenses)
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