ISSN:
1434-0879
Keywords:
COM crystal growth
;
Pyrophosphate
;
Citrate
;
Rat urine
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary An assay system for the measurement of the rate of Calcium Oxalate Monohydrate (COM) seed crystal growth in a metastable solution of calcium chloride and sodium oxalate containing traces of 14C-oxalic acid was used to assess the inhibitory activity of pyrophosphate (10−5 M-10−4 M), citrate (10−4 M-10−3 M) and urines of normal and pyridoxine deficient rats. Both pyrophosphate and citrate were strong inhibitors of COM crystal growth and caused a 50% decrease in crystal growth rate at 1.50×10−5 M and 2.85×10−4 M respectively. Normal rat urine strongly inhibited the COM crystal growth, while pyridoxine deficient animals showed a significant (p〈 0.01) decrease in mean inhibitory activity as compared to pair-fed controls. A lowered urinary inhibitory potential accompanied with hyperoxaluria and hypercalciuria, which is known to be associated with pyridoxine deficiency, may be a contributory risk of calcium oxalate crystallization and stone formation.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00262379
Permalink
