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Polarized cytoplasmic movement and inhibition of saltations induced by calcium-mediated effects of microbeams in fungal hyphae (1986)

McKerracher, L. J. ; Heath, I. B.

New York, NY : Wiley-Blackwell

Cell Motility and the Cytoskeleton 6 (1986), S. 136-145

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ISSN: 0886-1544

Keywords: cytoplasmic movement ; microbeam ; Ca++ ; fungi ; saltatory movement ; cytoskeleton ; Life and Medical Sciences ; Cell & Developmental Biology

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Biology , Medicine

Notes: We have investigated the mechanisms that hyphae of the fungus Basidiobolus magnus use to accomplish bulk movement of their cytoplasm and saltatory organelle movements. When cells were irradiated with an ultraviolet microbeam, cytoplasmic contraction occurred. The posterior cytoplasm (toward the septum) always moved forward toward the irradiated area, whereas anterior cytoplasm (between the cell tip and target) never contracted back toward the site of irradiation. Thus, there is an intrinsic polarity in the cytoplasm. Irradiations also arrested saltatory movements. The effects of irradiation on both saltations and cytoplasmic movement appear to be mediated by Ca++. Chelating exogenous Ca++ before irradiation eliminated contractions and prevented the inhibition of saltations. Furthermore, the effects of irradiation could be duplicated by using the Ca++ ionophore A23187. We relate the present results to our previous report on the effects of irradiation on the cytoskeleton [McKerracher and Heath, 1986]. We conclude that two separate cytoskeletal networks exist in fungal cells, and that an actin-containing network generates bulk cytoplasmic movement.

Additional Material: 9 Ill.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1002/cm.970060211

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UV microirradiations elicit Ca2+-dependent apex-directed cytoplasmic contractions in hyphae (1992)

Jackson, Sandra L. ; Heath, I. B.

Springer

Protoplasma 170 (1992), S. 46-52

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ISSN: 1615-6102

Keywords: Cytoplasmic migration ; Tip growth ; Actin ; Calcium ; UV microirradiation

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Summary Polarized tip-ward cytoplasmic contractions were induced in hyphae ofSaprolegnia ferax with ultraviolet microirradiations. These unidirectional contractions were similar in appearance and ionic requirements to those previously induced in hyphae ofBasidiobolus magnus, suggesting that the observed inherent cytoplasmic polarity is a general phenomenon. During growth the cytoplasm is continually moving forward with respect to the lateral cell wall and plasma membrane in order to maintain its position in the tip. These contractions may be an exaggerated form of this cytoplasmic migration. F-actin was most concentrated in the contracted cytoplasm, implying that it may be involved in generating the contraction. Contractions were enhanced by external Ca2+ and by irradiating the tip region which is rich in Ca2+ sequestering organelles, suggesting that flooding of the cytoplasm with Ca2+ caused the contractions. H+ did not affect contraction frequency. Neither the change in cytoplasmic consistency that preceded contraction, the contraction itself, nor the F-actin damage induced were confined to the microirradiated zone. This is in keeping with irradiation-induced damage to a network under tension or a flux of diffusible ions causing the response. Thus Ca2+ may regulate actin-myosin interactions that generate cytoplasmic migration.