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Further studies on the effects of myosin P-light chain phosphorylation on contractile properties of skinned cardiac fibres (1986)

Morano, I. ; Arndt, H. ; Bächle-Stolz, C. ; [et al.]

Springer

Basic research in cardiology 81 (1986), S. 611-619

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ISSN: 1435-1803

Keywords: myosin light chain kinase ; myosin P-light chain phosphorylation ; Ca2+-sensitivity ; skinned cardiac fibres ; unloaded shortening velocity

Source: Springer Online Journal Archives 1860-2000

Topics: Medicine

Notes: Summary We investigated the influence of myosin P-light chain phosphorylation by Ca2+-calmodulin dependent myosin light chain kinase (MLCK) on the sensitivity of the tension-pCa relation and maximum unloaded shortening velocity (v max) of chemically skinned heart fibres of the pig. Submaximum Ca2+ stimulation (pCa 5.5) induced 20±5% of the isometric tension achieved at maximum Ca2+ activation (pCa 4.3). MLCK-induced myosin P-light chain phosphorylation increased the isometric force development at pCa 5.5 by 40% whereas maximum tension at pCa 4.3 was not affected. Unloaded shortening velocity (v max) was not altered by myosin P-light chain phosphorylation either at maximum or at submaximum Ca2+ concentration, being c. 1.2 muscle length/s at pCa 5.5 and 2.2 muscle length/s at pCa 4.3. The MLCK-induced increase of the myosin P-light chain phosphorylation level was evaluated by determination of32P-incorporation. Two phosphorylatable myosin P-light chains could be demonstrated.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF02005185

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Zu Problemen der Photosynthesemessungen bei der Kultivierung von Mikroalgen (1985)

Arndt, H. ; Tschirner, E. ; Böhm, H.

Berlin : Wiley-Blackwell

Acta Biotechnologica 5 (1985), S. 35-44

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ISSN: 0138-4988

Keywords: Life Sciences ; Life Sciences (general)

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Process Engineering, Biotechnology, Nutrition Technology

Notes: The rate of photosynthetic activity of a single alga cell, their variability in the course of individual life, the time dependence of conditional influences, the dependence of the action of the condition on the registered parameter are shown by results of our measurements. The further underline the necessity of parallel short- or longtime measurements with several charges. Under these conditions, techniques on the basis of gasanalysers are profitable for the planning of measuring systems for photosynthesis with the ability to continuous, simulataneous in vivo measurements of photosynthetic rates of O2-output and CO2-fixation in open gas-stream with parallel charges. A measuring system, which meets these requirements, is demonstrated.

Additional Material: 7 Ill.