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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Clinical and experimental medicine 148 (1968), S. 72-80 
    ISSN: 1591-9528
    Keywords: Vital microscopy ; Fluorescence microscopy ; Capillary permeability ; Interstitial fluid ; Hamster cheek pouch ; Vitalmikroskopie ; Fluorescenzmikroskopie ; Gefäßpermeabilität ; Interstitielle Flüssigkeit ; Hamsterbackentasche
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Durch die lokale Injektion von Fluorescenzfarbstoffen in das lockere Bindegewebe der hervorgestülpten Hamsterbackentasche wurden Ausbreitung und Abtransport im Gewebe vitalmikroskopisch untersucht. Das frei wasserlösliche Fluorescein breitet sich in Gestalt perivasaler Streifen schnell in Richtung zur Basis der Backentasche hin aus, ohne dabei in das Blut überzutreten. Lymphgefäße gelangen nicht zur Darstellung. Das sich in vivo an Protein bindende Brillantsulfoflavin zeigt den streifenförmigen Abstrom verzögert und spärlich. Das in vitro an Plasmaproteine fest gekoppelte Fluorescein-Isothiocyanat bleibt während einer Beobachtungszeit von mehr als 30 min ohne Ausbreitung und Abstrom im Gewebe liegen. Der Stofftransport wasserlöslicher Substanzen im interstitiellen Gewebe der Hamsterbackentasche ist somit gerichtet und perivasal lokalisiert. Ein Abstrom über Lymphgefäße fehlt. Plasmaproteine bleiben im extravasalen Raum lange Zeit liegen.
    Notes: Summary The spread and flow of fluorescent dyes injected into the wall of the hamster cheek pouch was examined by vital microscopic means. Water-soluble fluorescein spreads very quickly in the form of perivascular streaks in the direction of the base of the cheek pouch, without entering into the blood stream. Lymph vessels, however, could not be identified. Brillant-sulfoflavine which binds proteinin vivo showed much slower and reduced flow phenomena. Fluorescein isothiocyanate coupled to plasma proteinsin vitro remained, during an observation period of more than 30 min, stationary in the cheek pouch tissue without any evidence of transport to be seen. The transport of water-soluble substances in the cheek pouch tissue is thus perivascular in nature. Lymph flow is not to be documented by this technique. This is further confirmed by the observation that fluorescent-labelled plasma proteins remain unmoved in the extravascular space of the cheek pouch tissue for a long period of time.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Research in experimental medicine 184 (1984), S. 1-15 
    ISSN: 1433-8580
    Keywords: Capillary permeability ; Intravital microscopy ; Fluorescence ; Perivascular diffusion ; TV microscope
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The transport of fluorescent tracer molecules of various molecular weights (MW 340-300,000) within the blood vessels and through the vessel walls into the perivascular tissue of the rat mesentery was studied microscopically. Using a highly sensitive TV-tube the fluorescence intensities were transformed into video signals and recorded for subsequent analysis. The results show that small molecules, such as the water-soluble dye fluorescein-sodium (FlNa) (MW 340) can pass through the wall of all blood vessels with minimal delay, whereas the passage of large molecules, such as the serum proteins (MW 〉 60,000), depends very strongly on the diameter of the vessels and on the medium in which the mesentery is embedded during the observation. In this respect, no difference was registered between vessels of the arterial and of the venous part of the microcirculation. The large serum protein molecules moved through the blood vessel walls at specific leakage points. The small dye molecules, however, traversed the wall uniformly along the entire length of the blood vessel. The small-molecule behavior could be described by a passive diffusion model with a cylindrical diffusion source.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
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