ISSN:
1040-0397
Keywords:
Glucose oxidase
;
Glucose
;
Carbon paste
;
Methylene-green mediator
;
Enzyme electrode
;
Chemistry
;
Polymer and Materials Science
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
An amperometric glucose bioelectrode based on the incorporation of methylene green (MG) within a glucose oxidase (GOD)-containing carbon paste is described. The electrode generates an anodic current in the range of 0.0-0.6 V (vs. SCE). The optimum pH is 8.5. The apparent Michaelis constant for the Cali bration plot is 49.3 mM (at 0.5% MG and 5.0% GOD loadings). The electrode sensitivity is 18.1 μA/mM cm2. The electrode is insensitive to paracetamol and ethanol. At 0.068 V the response to ascorbic acid (40 μM) accounts for 2.4% of that of the normal physiological glucose level in blood. The performance was optimized by systematically altering the electrode composition and electrode potential. The electrode was used to measure glucose in a serum sample and gave an acceptable correlation with the reference method Eksan-G results. Good stability was observed for storage in the dry state in the refrigerator.
Additional Material:
5 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/elan.1140070112
Permalink
