ISSN:
1615-6102
Keywords:
RuBisCO
;
Immunolocalization
;
Cyanobacteria
;
Plectonema boryanum
;
Carboxysomes
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Antiserum against the Calvin cycle enzyme, ribulose-1,5-bisphosphate carobxylase/oxygenase (RuBisCO), was used in conjunction with colloidal gold to localize RuBisCO in nitrogen-fixing (fix+) and nonfixing (fix−)Plectonema boryanum cells. RuBisCO antiserum consistently labeled the cytoplasm and polyhedral bodies (carboxysomes) in both fix+ and fix− cells. Through morphometry, it was determined that significantly less gold label (indicative of RuBisCO) was present in fix+ cells. This decreased RuBisCO content correlated with a decrease in net photosynthetic oxygen evolution also observed in fix+P. boryanum.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01666512
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