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  • Carotenoid biosynthesis, deficiency  (1)
  • Chloroplast development  (1)
Materialart
Erscheinungszeitraum
Schlagwörter
  • 1
    ISSN: 1432-2048
    Schlagwort(e): Carotenoid biosynthesis, deficiency ; Chlorophyll photooxidation ; Chloroplast envelope ; Mutant (Zea) ; Norflurazon ; Zea (carotenoid synthesis)
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Plastid-envelope membranes from seedlings ofZea mays L. made carotenoid-deficient by either norflurazon treatment or mutation lack an activity permitting conversion of phytoene to β-carotene. This activity in membrane fractions was measured by coincubation in vitro with a soluble system from spinach chloroplasts capable of converting [14C]isopentenyl pyrophosphate into phytoene. When grown in light, the carotenoid-deficient seedlings lack many soluble chloroplast proteins, including NADP-dependent malic enzyme (EC 1.1.1.40), pyruvate phosphate dikinase (EC 2.7.9.1), and ribulose-1,5-bisphosphate carboxylase (EC 4.1.1.39), but apparently still contain the soluble activities permitting synthesis of phytoene.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Springer
    Molecular genetics and genomics 252 (1996), S. 216-220 
    ISSN: 1617-4623
    Schlagwort(e): iojap ; Chloroplast development ; Transposon ; Robertson'sMutator ; Zea mays
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The recessive nuclear mutationiojap (ij) in maize produces striped plants with normal chloroplasts in green sectors and poorly developed chloroplasts in the white sectors. Theij mutation is also characterized by an array of additional phenotypic affects which suggests a pivotal role forIj in chloroplast development. TheIj gene from maize has been isolated; however, the sequence has not provided information on the action of theIj gene product. As an extension of the analysis of theIj gene we have generated a number of independent transposon insertion mutations using the Robertson'sMutator (Mu). Insertions were found to be distributed throughout the 5′ half of the gene and all showed a similar striping pattern to that of the originalij mutation,ij-ref. While several of the new mutant alleles producedij-homologous transcripts none produced detectable levels of the Ij polypeptide product. Chloroplast development in some cells appears to be able to proceed in the absence of the Ij polypeptide.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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