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  • 1
    Electronic Resource
    Electronic Resource
    Bovine adrenal medulla: Subcellular distribution of newly synthesised catecholamines, nucleotides and chromogranins (1972)
    Springer
    Naunyn-Schmiedeberg's archives of pharmacology 273 (1972), S. 43-61 
    Details
    ISSN: 1432-1912
    Keywords: Adrenal Medulla ; Chromaffin Granules ; Synthesis ; Catecholamines ; Nucleotides ; Chromogranins
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary 1. The subcellular distribution of newly synthesised catecholamines, nucleotides and proteins was investigated in bovine adrenal medulla. 3H-tyrosine, 3H-leucine and 32P-phosphate were used as radioactive precursors (“pulse label”). 2. Already 3 min after infusion of 3H-tyrosine the bulk of the labelled catecholamines was present in the “large granules” (mitochondria, lysosomes and chromaffin granules). In the fractions from the density gradient the distribution of the labelled and the total catecholamines was the same. Analogous results were obtained at longer time intervals. 3. 3 min after infusion of 32P-phosphate the labelled nucleotides present in the “large granules” were concentrated in the mitochondrial fraction. At longer time intervals after infusion of 32P-phosphate (45 min and 4h) chromaffin granules had accumulated a larger portion of the labelled nucleotides, mitochondria contained less. 4. After infusion of 45Ca2+, the isotope present in the large granules was found to be concentrated in the mitochondria and in chromaffin granules. 5. After infusion of 3H-leucine the soluble proteins of the adrenal medulla rapidly became labelled. 4 h after 3H-leucine newly synthesised proteins could be demonstrated in a particle which was present in the large granule fraction and which equilibrated in density gradients in a position corresponding to 1.6 M sucrose. This particle can be differentiated from mitochondria, microsomes, lysosomes and the bulk of the chromaffin granules. The labelled soluble proteins of this particle were identified as chromogranins. It seems likely that this particle represents a newly formed chromaffin granule which differs in its properties from the bulk of mature granules. The membrane proteins of this particle were not significantly labelled. 6. After infusion of 32P-phosphate the phospholipids of the adrenal medulla became labelled. The subcellular distribution of these phospholipids was similar to that of a microsomal marker enzyme, glucose-6-phosphatase. Lysoclecithin was not significantly labelled. 7. In the light of these results the subcellular events leading to the formation of complete chromaffin granules are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00508079
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  • 2
    Electronic Resource
    Electronic Resource
    Bovine adrenal medulla: Synthesis and secretion of radioactively labelled catecholamines and chromogranins (1971)
    Springer
    Naunyn-Schmiedeberg's archives of pharmacology 271 (1971), S. 193-203 
    Details
    ISSN: 1432-1912
    Keywords: Adrenal Medulla ; Chromogranin ; Catecholamines ; Synthesis ; Secretion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary 1. 3H-leucine and3H-tyrosine were added to the perfusion medium of isolated bovine adrenal glands in order to study the synthesis and secretion of radioactively labelled catecholamines and proteins. 2. Various times after the injection of the labelled amino acids the soluble proteins of the microsomal fraction were isolated and subjected to polyacrylamide gel electrophoresis in three different buffer systems. The distribution of protein-bound radioactivity within the gels was determined. Most of the label was found to be confined to proteins which behaved like the specific soluble proteins of chromaffin granules, i.e. the chromogranins. 3. Stimulation of the adrenal gland with carbachol induced a release of catechol-amines, protein, and protein-bound radioactivity. In the absence of Ca2+ the secretion of all these components was abolished. The highest specific radioactivity of the proteins secreted upon stimulation was reached 4 h after the injection of the labelled precursors. The labelled proteins, secreted upon stimulation, could be identified as chromogranins. 4. Carbachol induced the release of highly labelled catecholamines after the injection of3H-tyrosine. The highest specific radioactivity of these catecholamines was already observed at the first stimulation with carbachol i.e. 30 min after3H-tyrosine. 5. These results demonstrate that in isolated bovine adrenal glands radioactively labelled chromogranins and catecholamines can be synthesised and can be secreted upon stimulation with carbachol.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00998580
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  • 3
    Electronic Resource
    Electronic Resource
    Darstellung und röntgenographische Untersuchung des Zinkazidtrihydrates Zn(N3)2 · 3 H2O (1975)
    Weinheim : Wiley-Blackwell
    Zeitschrift für anorganische Chemie 413 (1975), S. 94-96 
    Details
    ISSN: 0044-2313
    Keywords: Chemistry ; Inorganic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Preparation and X-ray Investigation of Zinc Azide TrihydrateZn(N3)2 · 3 H2O has been crystallized from an aqueous solution of zinc azide. Single crystals, which are unstable in air, were investigated my means of x-ray techniques. The lattice parameters are: a = 9.95 Å, b = 5.94 Å, c = 14,30 Å, β = 134.55°, N = 4. Indexd powder pattern are listed.
    Notes: Zn(N3)2 · 3 H2O wurde aus wäßriger Zinkazidlösung hergestellt. Die röntgenographische Untersuchung der an Luft unbeständigen Einkristalle ergab folgende Gitterkonstanten: a = 9,95 Å, b = 5,94 Å, c = 14,30 Å, β = 134,55°, N = 4. Indizierte Pulverdaten wurden tabelliert.
    Additional Material: 2 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/zaac.19754130112
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  • 4
    Electronic Resource
    Electronic Resource
    Stereoisomerie bei inneren Komplexsalzen. (Stereoisomere Kobaltisalze der α-Aminosäuren.) (1912)
    Weinheim : Wiley-Blackwell
    Berichte der deutschen chemischen Gesellschaft 45 (1912), S. 372-377 
    Details
    ISSN: 0365-9496
    Keywords: Chemistry ; Inorganic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/cber.19120450155
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  • 5
    Electronic Resource
    Electronic Resource
    Über Stereoisomerie bei inneren Komplexsalzen (1909)
    Weinheim : Wiley-Blackwell
    Berichte der deutschen chemischen Gesellschaft 42 (1909), S. 3894-3902 
    Details
    ISSN: 0365-9496
    Keywords: Chemistry ; Inorganic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/cber.190904203144
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  • 6
    Electronic Resource
    Electronic Resource
    Über Salzbildung bei Oxy-guanidinen. (Über innere Komplexsalze. XII) (1914)
    Weinheim : Wiley-Blackwell
    Berichte der deutschen chemischen Gesellschaft 47 (1914), S. 2945-2947 
    Details
    ISSN: 0365-9496
    Keywords: Chemistry ; Inorganic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/cber.19140470389
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    Paper (German National Licenses)
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