ISSN:
1432-1912
Schlagwort(e):
Extraneuronal monoamine oxidase
;
Uptake2
;
Rat heart
;
Extraneuronal deaminating system
;
Catecholamines
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Medizin
Notizen:
Summary The handling of five amines by the extraneuronal deaminating system was studied in perfused hearts of rats (pretreated with reserpine; COMT and neuronal uptake inhibited). Hearts were perfused with 50 nmol/l 3H-noradrenaline for 30 min, in the presence of increasing concentrations of unlabelled (−)-adrenaline, (−)-noradrenaline, dopamine, tyramine and 5-HT. IC50's were determined as those concentrations of unlabelled amines which halved the steady-state rate of deamination of 3H-noradrenaline. After correction for changes in the tissue/medium ratio for 3H-noradrenaline, “half-saturating outside concentrations” were obtained. They increased in the order (−)-adrenaline (15 μmol/l) — tyramine — dopamine — noradrenaline —5-HT (53 μmol/l). The V max for extraneuronal deamination was determined for 3H-(−)-adrenaline, 3H-(−)-noradrenaline and 3H-dopamine, as well as (by HPLC and electrochemical detection) for tyramine and 5-HT. It was low for (−)-adrenaline, intermediate for (−)-noradrenaline, dopamine and 5-HT, high for tyramine. For the three catecholamines the half-saturating outside concentrations of the extraneuronal deaminating system clearly exceeded those for the extraneuronal O-methylating system of the same organ (see Grohmann and Trendelenburg 1985), although the two enzymes appear to co-exist in the same cells, so that the same transport system is involved.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1007/BF00169243
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