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  • 1
    Electronic Resource
    Electronic Resource
    Ist der Nachweis von Blutspuren durch 3-Amino-phthalsäurehydrazid ein kennzeichnendes Verfahren? (1941)
    Weinheim : Wiley-Blackwell
    Zeitschrift für die chemische Industrie 54 (1941), S. 213-215 
    Details
    ISSN: 0044-8249
    Keywords: Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ange.19410541703
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  • 2
    Electronic Resource
    Electronic Resource
    Syntheses and Structures of Bis(aryloxo)fluoroytterbium(III) Complexes, [Yb(OAr)2F(THF)]2 (OAr = OC6H2-2,6-tBu2-4-R; R = H, Me, tBu), and Bis(cyclopentadienyl)fluoroytterbium(III) Complexes, [YbCp2F]3, [Yb(MeCp)2F]4, [YbCp2F(OPPh3)]2, and [Yb(MeCp)2F(THF)]2 (2000)
    Weinheim : Wiley-Blackwell
    Berichte der deutschen chemischen Gesellschaft 2000 (2000), S. 1061-1071 
    Details
    ISSN: 1434-1948
    Keywords: Lanthanide(III) ; Ytterbium ; Aryloxides ; Fluorine ; C-F Activation ; Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Reaction of [Yb(OAr)2(THF)3] (OAr = OC6H2-2,6-tBu2-4-R; R = H, Me, tBu) with perfluorodecalin in THF at room temperature results in C-F activation and formation of the first heteroleptic aryloxofluorolanthanoid complexes, [Yb(OAr)2F(THF)]2. Oxidation of bis(cyclopentadienyl)ytterbium(II) with perfluoro(methylcyclohexane) or perfluorodecalin in DME surprisingly gives unsolvated [YbCp2F]3. The analogous reaction of bis(methylcyclopentadienyl)ytterbium(II) yields unsolvated [Yb(MeCp)2F]4, whilst in THF, the oxidation provides [Yb(MeCp)2F(THF)]2. Treatment of [YbCp2F(THF)]2 with triphenylphosphane oxide gives [YbCp2F(OPPh3)]2. X-ray structure determinations revealed [Yb(OAr)2F(THF)]2 (R = H or tBu) to be centrosymmetric fluoride-bridged dimers with five-coordination for ytterbium. Examination of the structures of the cyclopentadienyl complexes showed that [YbCp2F]3 is trimeric with formal eight-coordination for ytterbium and a planar (YbF)3 ring, whereas [Yb(MeCp)2F]4 is an eight-coordinate tetramer having a puckered (YbF)4 ring with F-Yb-F angles of ca. 90° and Yb-F-Yb angles close to 180° [178.9(4), 168.4(3)°]. Both [Yb(MeCp)2F(THF)]2 and [YbCp2F(OPPh3)]2 are nine-coordinate fluoride-bridged dimers.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Borane Insertion into Group-4 Metal-to-Carbon Bonds: The Reaction of HB(C6F5)2 with (η2-Formaldehyde)zirconocene Dimer and with (Butadiene)zirconocene (1999)
    Weinheim : Wiley-Blackwell
    Berichte der deutschen chemischen Gesellschaft 1999 (1999), S. 2243-2247 
    Details
    ISSN: 1434-1948
    Keywords: Metallaoxirane ; HBR2 addition ; (Formaldehyde)zirconocene ; (Butadiene)zirconocene ; Heterocycles ; Boron ; Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: (η2-Formaldehyde)zirconocene dimer (8) cleanly adds one or two molar equivalents of the borane HB(C6F5)2 by insertion of the H-[B] unit into the zirconium-carbon bond of the metallaoxirane moieties to form the mono- and bis-insertion products 16 and 17, respectively. These systems contain five-membered heterocyclic rings that are built up by connecting five different elements, namely H, B, C, O, and Zr. The bis(borane) insertion product 17 was characterized by an X-ray crystal structure analysis. (Butadiene)zirconocene reacts with HB(C6F5)2 in a similar way by insertion of the H-[B] unit into the (butadiene)C4-Zr linkage to form the metallacycle 18.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Association of the β isoform of protein kinase C with vimentin filaments (1992)
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 22 (1992), S. 250-256 
    Details
    ISSN: 0886-1544
    Keywords: cytoskeletal localization ; signal transduction ; intermediate filaments ; rat basophilic leukemia cells ; translocation ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Protein kinase C (PKC) isoforms are key mediators in hormone, growth factor, and neurotransmitter triggered pathways of cell activation (Nishizuka: Science 233:305-312, 1986; Nature 334:661-665, 1988). Stimulation of kinase activity by diacylglycerol and calcium often leads to translocation of PKC from the cytosol to a particulate fraction (Kraft and Anderson: Nature 301:621-623, 1983). The β isoform of PKC is translocated and degraded much more rapidly than the β isoform in phorbolester-stimulated rat basophilic leukemia (RBL) cells (Huang et al.: J. Biol. Chem. 264:4238-4243, 1989). We report here immunofluorescence evidence that the distributions of PKC α and β are strikingly different in antigen-activated RBL cells. PKC β associates with perinuclear filaments and filaments that extend from the perinuclear area to the cell periphery whereas PKC β concentrates in regions of the cell periphery. This distribution of PKC β is distinctly different from that of actin filaments and microtubules as determined by phalloidin staining and by anti-tubulin antibody labeling. In contrast, the staining patterns obtained with antibodies to PKC β and to the intermediate filament protein vimentin are almost identical, indicating that PKC β associates with vimentin filaments. These bundles of 100 Å filaments may provide docking sites for interactions of PKC β with its substrates and thus confer specificity to the actions of this isoform. © 1992 Wiley-Liss, Inc.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/cm.970220405
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  • 5
    Electronic Resource
    Electronic Resource
    Tubulin gene expression during growth and maturation of leaves with different developmental patterns (1995)
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 30 (1995), S. 67-72 
    Details
    ISSN: 0886-1544
    Keywords: Nicotiana ; Hordeum ; microtubule ; cell differentiation ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Changes in the tubulin-protein and -poly(A)+RNA contents were monitored by means of Western and Northern blot analyses, respectively, during growth and maturation of leaves of a dicotyledonous (tobacco) and monocotyledonous (barley) plant. It was recently argued from immunofluorescence and preliminary biochemical data that the density of microtubular networks and concomitantly the tubulin content are distinctly reduced after cessation of cell growth in leaves [Jung et al., 1993]. The results presented now confirm and extend this view. There appeared to be clear differences between the monocot and the dicot: (1) the loss of tubulin during leaf development was much slower in the dicot than in the monocot leaves (within months instead of days); (2) the degree of loss was more dramatic in the monocot leaf and only very low threshold levels of tubulin were retained in fully differentiated tissues; and (3) the loss of tubulin in the monocot leaf tissue appeared to be correlated with the decrease in the mRNA content, whereas the high level of tubulin-RNA in fully differentiated or even almost senescent dicot leaves indicated a gene expression control at the posttranscriptional level.The comparatively rapid and very distinct tubulin-protein and -RNA disappearance during development of the monocot leaf tissues confirm at the molecular level that differentiation proceeds much faster and is much more determinative in these leaves, as was postulated from histological and physiological data. The differences in the behaviour of the microtubular cytoskeleton perhaps even reflect the differences in the ability of the differentiated leaf cells to dedifferentiate, i.e., to establish new sets of microtubules and to reenter the mitotic cell cycle, e.g., during would response, tumour induction or in vitro culture. © 1995 Wiley-Liss, Inc.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/cm.970300108
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  • 6
    Electronic Resource
    Electronic Resource
    Corpus luteum growth and plasma progesterone levels in unilaterally ovariectomized pregnant rats (1979)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 195 (1979), S. 311-316 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Unilateral ovariectomy (ULO) was carried out on rats at Day 8 of gestation: term is Day 23. The effect on peripheral plasma progesterone levels at Day 16 and on growth and cellular changes in the remaining corpora lutea (CL) at Day 17 was determined by comparison with results from control and sham-operated rats. ULO increased the volume of remaining CL by 18% at Day 17. At Day 16 this effect was only apparent in rats with three or fewer CL remaining. CL hypertrophy was due to an increase in the volume of individual luteal cells and not to cell hyperplasia. Luteal cell nuclear volume also increased as did the number of endothelial cells per corpus luteum. The proportion of the corpus luteum occupied by vascular space did not alter.ULO had little effect on mean plasma progesterone levels at Day 16 (92 ± 7 ng/mltreated; 86 ± 11 ng/ml controls). In rats with three or fewer CL remaining, however, progeserone levels were low (51 ± 7 ng/ml).It was concluded that ULO can enhance the growth of corpora lutea and perhaps increase their rate of progesterone production.
    Additional Material: 3 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1091950206
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  • 7
    Electronic Resource
    Electronic Resource
    Cytological events associated with in vitro aged and fertilized rabbit eggs (1979)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 195 (1979), S. 357-374 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The morphogenetic events associated with rabbit eggs aged in vitro for 12 to 50 hours prior to mixing with sperm have been examined by light and electron microscopy. After 12 hours in culture, morphological alterations of the meiotic spindle and the cortex of unfertilized eggs were evident. By 24 to 50 hours in culture, unfertilized eggs contained subnuclei, structures which formed when individual and/or groups of meiotic chromosomes dispersed and became invested by a double-laminated structure reminiscent of a nuclear envelope.Although most eggs obtained 11.5 to 12 hours after induced ovulation and in vitro fertilized displayed morphogenetic patterns similar to those described for in vivo fertilized ova, some (10%) contained three pronuclei. Many eggs obtained 13 to 15 hours after induced ovulation and subsequently mixed with sperm in vitro appeared to undergo processes of fertilization typical of in vivo fertilized eggs, however, approximately 30% contained subnuclei in association with the male pronucleus. Few eggs (15%) aged 12 hours prior to in vitro fertilization displayed patterns of pronuclear development and association typical of fertilized unaged ova. Subnuclei developed in many of the fertilized ova. Supernumerary sperm nuclei, which did not develop into male pronuclei, were observed in some zygotes. Cleavage of eggs aged 12 hours prior to fertilization was abnormal or retarded. After 24 hours in culture approximately 16% of the eggs fertilized. Seventy percent of the fertilized eggs failed to Support the development of a male or female pronucleus.
    Additional Material: 3 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1091950209
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  • 8
    Electronic Resource
    Electronic Resource
    Quantitative cell changes and vascularisation in the early corpus luteum of the pregnant rat (1980)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 197 (1980), S. 369-374 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Early corpora lutea (CL) of the rat were histologically examined on Day 1, 2, 4, and 6 of gestation. Measurements were taken of total volume and of the number of luteal and endothelial cells in one CL of both ovaries of five rats at each stage examined. CL volume increased over the 6 days from --0.76 to 1.39μl and peripheral plasma progesterone levels from 8.1 to 33.2 ng/ml. The number of luteal cells per CL (range 303,000 to 37,000) did not significantly change, and there was no evidence of mitosis or death amongst these cells. Luteal cell volume increased from 1.74 to 3.49 pl and nuclear volume from 0.25 to 0.38 pl, the former being the major cause of CL growth. The CL appeared to be richly vascularized, even on Day 1, and the number of endothelial cells per CL (range 289,000 to 354,000) remained relatively constant over the period examined.It was concluded that the number of luteal cells per CL is determined prior to or around ovulation in the rat and that subsequent growth of the CL is due to hypertrophy and not hyperplasia.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1091970311
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  • 9
    Electronic Resource
    Electronic Resource
    The cellular pattern of corpus luteal growth during pregnancy in the rat (1979)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 193 (1979), S. 823-830 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The cellular pattern of corpus luteal (CL) growth was studied in rats at Days 6, 10, 12, 14, 16, 17 and 22 of pregnancy: term is Day 23. Measurements were taken of the percentage of the CL occupied by luteal cells, connective tissue and vascular space, luteal cell and nuclear volumes, and the number of luteal and endothelial cells in each of three CL in both ovaries of five rats at each stage of pregnancy. Total CL volume increased from 1.08-3.23 μl over Days 10-17. This was mainly due to an increase in luteal cell volume from 3.72 pl to 9.30 pl. Neither the number of luteal cells per CL (range 212,000-287,000) nor the percentage of the CL occupied by luteal cells (range 85-90%) had much influence on growth. Nuclear volume increased roughly in proportion to cytoplasmic volume but near term it decreased despite little change in cytoplasmic volume. The number of endothelial cells per CL increased steadily from 398,000 at Day 6 to 1,545,000 at Day 22. There was a strong negative correlation (r = -0.78) between the number of luteal cells per CL and mean luteal cell volume that was evident at all stages of pregnancy. There was a positive, but weaker correlation (r = 0.35) between number of luteal cells and CL volume. Thus, CL volume seems to be partly determined by the number of luteal cells at Day 6 but this effect is moderated by local control of luteal cell volume.
    Additional Material: 2 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1091930406
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  • 10
    Electronic Resource
    Electronic Resource
    Ultrastructural changes of rat blastocysts induced by estrogen during delayed implantation (1974)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 179 (1974), S. 253-271 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Ultrastructural changes of rat blastocysts during delayed implantation were studied 16, 20, 24 or 30 hours after estrogen was given to induce implantation.In the inner cell mass the presence of long cytoplasmic processes penetrating deeply into the neighboring inner cell mass cells is seen at 16 hours. Most cells also show an increased number of ribosomes, polyribosomes and granular endoplasmic reticulum.The trophoblast is featured by the formation of large amounts of glycogen and many inclusion bodies. Glycogen granules appear first in some abembryonic trophoblast cells at 16 hours, and spread to the embryonic pole at 24 hours. New inclusion bodies appear sequentially: multivesicular bodies at 20 hours, multigranular bodies at 24 hours and lamellar bodies at 30 hours. The functions of these inclusion bodies remain to be studied.
    Additional Material: 1 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1091790208
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