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  • Polymer and Materials Science  (7)
  • Cell & Developmental Biology  (2)
  • Pisum sativum  (2)
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  • 1
    ISSN: 1615-6102
    Schlagwort(e): Calcium ; Cell elongation ; Indole-3-acetic acid ; Pisum sativum ; Secretion
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary The treatment of dark grown pea stem segments with chelators of divalent cations (EGTA, EDTA, CTC), various Ca2+ antagonists (LaCl3, A-23187, verapamil) and inhibitors of secretory processes (monensin, CB) reduced elongation in the presence of indole-3-acetic acid (IAA). Generally the inhibition increased with increasing concentrations of the substances. The timing of the responses can be correlated with maximum auxin-stimulated secretion of cell wall material. Examination of cell ultrastructure showed that changes in dictyosome activity could explain a reduced deposition of cell wall material and so cause inhibition of elongation. The inhibitors affected the morphology and vesiculation of the dictyosomes, and the appearance of the plasma membrane, ER and mitochondria in different ways. The most pronounced effects on ultrastructure resulted from monensin and LaCl3 treatments with the dictyosomes being most affected; large vesicles appeared in the cytoplasm. Less pronounced effects on cell structure were seen in EGTA, A-23187 and verapamil treated tissue. The effects on the dictyosomes are considered to be due to disturbances of Ca2+ and other ionic levels within the cells.
    Materialart: Digitale Medien
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  • 2
    ISSN: 1615-6102
    Schlagwort(e): Dictyosomes ; Indoleacetic acid ; Pisum sativum ; Stereology
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary A quantitative analysis of electron micrographs showed that IAA treatment caused an initial rapid increase in the amount of dictyosome material in pea stem epidermal cells. The increase was detected within 15 minutes of auxin presentation and reached a maximum around 30 minutes. This was followed by a decrease, presumably due to an increased utilization of the organelle. The decrease involved a fall in the amount of dictyosome-derived vesicles and in the actual number of dictyosomes. The results are discussed in relation to similar observations on expanding cells of monocotyledonous plants.
    Materialart: Digitale Medien
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  • 3
    Digitale Medien
    Digitale Medien
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 180 (1974), S. 597-603 
    ISSN: 0003-276X
    Schlagwort(e): Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Medizin
    Notizen: The minimum period of uterine exposure required by ejaculated boar spermatozoa as a preliminary to rapid capacitation has been determined after natural or surgical deposition of sperm samples directly into the uterine lumen. Twenty-four oestrous gilts were mated or inseminated close to the time of ovulation, and 15, 30, 45 or 60 minutes later, the Fallopian tubes were separated from the uterine cornua. The tubes were flushed at pre-arranged intervals during a second intervention, and the proportion of eggs penetrated and activated examined by phase-contrast microscopy.On the basis of 166 eggs recovered from eighteen mated gilts, a period of uterine exposure as brief as 30 minutes, when followed by a tubal residence of approximately three hours, permitted 30.3% of the eggs to be activated; this proportion increased to 51.6% and 60.5% if the tubes were isolated 45 or 60 minutes, respectively, after mating (p 〈 0.001), as did the mean number of spermatozoa associated with the eggs. When the cornua were separated from the tubes 15 minutes after semen deposition into the uterus of six animals, 11.3% of 62 eggs were fertilized during the ensuing three and one half hours, but very few spermatozoa had reached and/or attached to the eggs in this group.It is concluded that a population of boar spermatozoa potentially capable of effecting fertilization may enter the tubes within 15 to 30 minutes of mating near the time of ovulation, and that such vanguard spermatozoa can activate a proportion of the eggs within a further two to three hours. Thus, from a temporal point of view, the major components of the capacitation process in oestrous pigs are inferred to take place in the Fallopian tubes.
    Zusätzliches Material: 1 Tab.
    Materialart: Digitale Medien
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  • 4
    Digitale Medien
    Digitale Medien
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Polymer Science 13 (1969), S. 2085-2096 
    ISSN: 0021-8995
    Schlagwort(e): Chemistry ; Polymer and Materials Science
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Chemie und Pharmazie , Maschinenbau , Physik
    Notizen: Polyethylene, polypropylene, poly(vinyl fluoride) (Tedlar), polystyrene, nylon 6, poly(ethylene terephthalate) (Mylar), polycarbonate, cellulose acetate butyrate, and a poly(oxymethylene) copolymer were treated with activated helium and with activated oxygen. Mechanical strengths of adhesive-bonded specimens prepared from treated and from untreated coupons were compared. Polyethylene (PE) and polypropylene (PP) showed the greatest increases in bond strength. Oxygen and helium were both effective with polyethylene, but polypropylene showed no improvement when treated with activated helium. The results with excited helium parallel the effects of ionizing radiation on these two polymers, as does the appearance of unsaturation bands in the infrared (965 cm-1 in PE, and 887 and 910 cm-1 in PP). Active nitrogen produced excellent bond strength with polyethylene but not with polypropylene. Of the remaining polymers examined, Tedlar, polystyrene, and nylon 6 showed the greatest improvement in bondability after treatment, and Mylar showed moderate improvement. Polycarbonate, cellulose acetate butyrate, and the poly(oxymethylene) copolymer gave approximately two-fold increases in lap-shear bond strength. In several cases, significant differences in response to time of treatment and type of excited gas were found.
    Zusätzliches Material: 6 Tab.
    Materialart: Digitale Medien
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  • 5
    Digitale Medien
    Digitale Medien
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Polymer Science 16 (1972), S. 1465-1477 
    ISSN: 0021-8995
    Schlagwort(e): Chemistry ; Polymer and Materials Science
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Chemie und Pharmazie , Maschinenbau , Physik
    Notizen: The bondability of the following polymers as a function of length of exposure to excited helium or oxygen was investigated: low-density polyethylene, high-density polyethylene (two types), poly(4-methyl-1-pentene), poly(vinyl fluoride), poly(vinylidene fluoride), FEP Teflon, poly(oxymethylene) copolymer, nylon 6, nylon 66, poly(ethylene terephthalate), and polystyrene. Generally, the bond strength increase rapidly initially and then remains nearly constant, perhaps decreasing in some cases at long exposure times. A method is presented for calculating bond strength-versus-exposure time curves. The calculated curves generally fit the data reasonably well. Polypropylene showed a rapid increase in bondability with exposure to excited oxygen. Helium was ineffective toward this polymer under normal conditions, but could produce good bond strength at higher temperatures.
    Zusätzliches Material: 5 Ill.
    Materialart: Digitale Medien
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  • 6
    ISSN: 0021-8995
    Schlagwort(e): Chemistry ; Polymer and Materials Science
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Chemie und Pharmazie , Maschinenbau , Physik
    Notizen: Several studies of the influence of contact resistance on the measurement of thermal conductivity of polystyrene using a steady-state device are presented. In each case it is seen that, although this influence can perhaps be minimized, it cannot be eliminated as an experimental complication. A novel technique is then described which does eliminate entirely contact resistance as a variable, and this technique is shown to yield an unequivocal value of thermal conductivity.
    Zusätzliches Material: 8 Ill.
    Materialart: Digitale Medien
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  • 7
    ISSN: 0021-9541
    Schlagwort(e): Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Medizin
    Notizen: Mouse teratocarcinoma cells derived from embryoid bodies of 129SVsl mice were cultured in vitro to permit their differentiation. These cells were then infected with simiam virus 40 (SV40) and 31 cloned cell lines (SVTER) were derived from these cultures. All 31 SVTER cell lines contained the SV40 tumor (T) antigen and grew as permanent lines in culture. Mock-infected embryoid body cultures did not give rise to permanent cell lines. The morphology of each SVTER cell line was distinct and did not change during successive subclonings.The growth properties and tumorigenic potential of all 31 SVTER cell lines were investigated. None of these lines produced tumors in 129SVsl mice. Each cell line was tested for its ability to (1) grow in medium containing 1% serum, (2) plate on a cell monolayer, and (3) form clones in methocel suspension. Only three of the SVTER cell lines were transformed with respect to all three of these criteria. Most of these cell lines were minimal transformation.The SVTER cell lines were tested for creatine phosphokinase (CPK), an enzyme activity characteristic of mouse brain and muscle tissue, and the protease, plasminogen activator (PA) which is found in embryoid bodies and several differentiated cell types. Some of the SVTER cell lines contained high levels of CPK, while others had high levels of PA and a third group of cells contained neither enzyme activity. No SVTER cell line was found with high levels of both these enzyme activities. This result suggests that mutually exclusive sets of genes are expressed in these cells as might be expected from the distinct tissue distribution of the two enzyme activities studied. These SVTER cell lines may be useful in reconstructing developmental pathways of differentiating teratomas in vitro.
    Zusätzliches Material: 3 Ill.
    Materialart: Digitale Medien
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  • 8
    Digitale Medien
    Digitale Medien
    New York : Wiley-Blackwell
    Journal of Polymer Science: Polymer Chemistry Edition 19 (1981), S. 1357-1360 
    ISSN: 0360-6376
    Schlagwort(e): Physics ; Polymer and Materials Science
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Chemie und Pharmazie
    Notizen: 1,3-Butadiene was polymerized anionically to polybutadiene which contained up to 100% 1,2-addition product. The atactic 100% 1,2-polybutadiene was prepared with n-butyllithium modified with bis piperidino ethane. The polymerization was done in hexane solvent at -5 to +20°C polymerization temperature.
    Zusätzliches Material: 2 Tab.
    Materialart: Digitale Medien
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  • 9
    Digitale Medien
    Digitale Medien
    Hoboken, NJ : Wiley-Blackwell
    Journal of Polymer Science 57 (1962), S. 693-709 
    ISSN: 0022-3832
    Schlagwort(e): Chemistry ; Polymer and Materials Science
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Chemie und Pharmazie , Physik
    Notizen: Carbon blacks inhibit and retard free radical polymerization in bulk and solution. The mechanism of this is discussed in terms of the chemical groups present on the carbon surface. It is shown that the number of free radicals which react during the induction period (polymerization of styrene with AIBN) is much less than the number of quinone groups but of the same order of magnitude as the number of easily oxidizable groups. Inhibition by carbon black was also found in an emulsion polymerization system with cumene hydroperoxide as initiator (no reducing components). By use of highly active redox recipes, reasonable rates of polymerization were obtained at both 50°C. (sugar-iron-hydroperoxide recipe) and 5°C. (peroxide-polyamine recipe), with as much as 40 parts of black per 100 parts of monomers. In these systems, the carbon black was charged as an aqueous dispersion stabilized with Daxad 11; the latices were stable and showed evidence of association between some of the carbon black and some of the latex particles. The coagulated rubber stocks, when compounded and cured, gave properties closely similar to those of dry mixed stocks.
    Zusätzliches Material: 8 Ill.
    Materialart: Digitale Medien
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  • 10
    Digitale Medien
    Digitale Medien
    New York : Wiley-Blackwell
    Journal of Polymer Science Part B: Polymer Letters 4 (1966), S. 463-465 
    ISSN: 0449-2986
    Schlagwort(e): Chemistry ; Polymer and Materials Science
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Chemie und Pharmazie
    Materialart: Digitale Medien
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